ArticleFungal genetics and biology : FG & B2015
Fluorescent markers for the Spitzenkörper and exocytosis in Zymoseptoria tritici.
Article in Fungal genetics and biology : FG & B, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Article
- Functional characterization of extracellular and intracellular catalase-peroxidases involved in virulence of the fungal wheat pathogen Zymoseptoria tritici.Molecular plant pathology · 2024Article
- MoMih1 is indispensable for asexual development, cell wall integrity, and pathogenicity ofFrontiers in plant science · 2023Article
- Zymoseptoria tritici white-collar complex integrates light, temperature and plant cues to initiate dimorphism and pathogenesis.Nature communications · 2022Article
- Article
- Conditional promoters to investigate gene function during wheat infection by Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2021Article
- The fungicide dodine primarily inhibits mitochondrial respiration in Ustilago maydis, but also affects plasma membrane integrity and endocytosis, which is not found in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2020Article
- Class V chitin synthase and β(1,3)-glucan synthase co-travel in the same vesicle in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2020Article
- Phosphopantetheinyl transferase (Ppt)-mediated biosynthesis of lysine, but not siderophores or DHN melanin, is required for virulence of Zymoseptoria tritici on wheat.Scientific reports · 2018Article
- Fluorescent markers of various organelles in the wheat pathogen Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2017Article
- Cell Biology of Hyphal Growth.Microbiology spectrum · 2017Review
- The Small GTPase MoSec4 Is Involved in Vegetative Development and Pathogenicity by Regulating the Extracellular Protein Secretion inFrontiers in plant science · 2016Article
- The Exocyst Complex in Health and Disease.Frontiers in cell and developmental biology · 2016Review
- Taming a wild beast: Developing molecular tools and new methods to understand the biology of Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Fungal hyphae are highly polarized cells that invade their substrate by tip growth. In plant pathogenic fungi, hyphal growth is essential for host invasion. This makes polarity factors and secretion regulators potential new targets for novel fungicides. Polarization requires delivery of secretory vesicles to the apical Spitzenkörper, followed by polarized exocytosis at the expanding cell tip. Here, we introduce fluorescent markers to visualize the apical Spitzenkörper and the apical site of exocytosis in hyphae of the wheat pathogen Zymoseptoria tritici. We fused green fluorescent protein to the small GTPase ZtSec4, the myosin light chain ZtMlc1 and the small GTPase ZtRab11 and co-localize the fusion proteins with the dye FM4-64 in the hyphal apex, suggesting that the markers label the hyphal Spitzenkörper in Z. tritici. In addition, we localize GFP-fusions to the exocyst protein ZtExo70, the polarisome protein ZtSpa2. Consistent with results in the ascomycete Neurospora crassa, these markers did localize near the plasma membrane at the hyphal tip and only partially co-localize with FM4-64. Thus, these fluorescent markers are useful molecular tools that allow phenotypic analysis of mutants in Z. tritici. These tools will help develop new avenues of research in our quest to control STB infection in wheat.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.