ArticleFungal genetics and biology : FG & B2015
A codon-optimized green fluorescent protein for live cell imaging in Zymoseptoria tritici.
Article in Fungal genetics and biology : FG & B, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 62 citations in OpenAlex.
- Visualizing Oral Infection Dynamics ofJournal of fungi (Basel, Switzerland) · 2025Article
- Article
- The Egyptian wheat cultivar Gemmeiza-12 is a source of resistance against the fungus Zymoseptoria tritici.BMC plant biology · 2024Article
- Use of chitin:DNA ratio to assess growth form in fungal cells.BMC biology · 2024Article
- Sas3-mediated histone acetylation regulates effector gene activation in a fungal plant pathogen.mBio · 2023Article
- Zymoseptoria tritici white-collar complex integrates light, temperature and plant cues to initiate dimorphism and pathogenesis.Nature communications · 2022Article
- Asexual reproductive potential trumps virulence as a predictor of competitive ability in mixed infections.Environmental microbiology · 2022Article
- Construction of nucleus-directed fluorescent reporter systems and its application to verification of heterokaryon formation inFrontiers in microbiology · 2022Article
- Mixed infections alter transmission potential in a fungal plant pathogen.Environmental microbiology · 2021Article
- A wheat cysteine-rich receptor-like kinase confers broad-spectrum resistance against Septoria tritici blotch.Nature communications · 2021Article
- Conditional promoters to investigate gene function during wheat infection by Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2021Article
- Article
- Fluorescent protein expression in the ectomycorrhizal fungus Laccaria bicolor: a plasmid toolkit for easy use of fluorescent markers in basidiomycetes.Current genetics · 2020Article
- Morphological changes in response to environmental stresses in the fungal plant pathogen Zymoseptoria tritici.Scientific reports · 2019Article
- ATP prevents Woronin bodies from sealing septal pores in unwounded cells of the fungus Zymoseptoria tritici.Cellular microbiology · 2017Article
- Fluorescent markers of various organelles in the wheat pathogen Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2017Article
- Cell Biology of Hyphal Growth.Microbiology spectrum · 2017Review
- Cell biology of Zymoseptoria tritici: Pathogen cell organization and wheat infection.Fungal genetics and biology : FG & B · 2015Review
- Conditional promoters for analysis of essential genes in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
- Fluorescent markers for the Spitzenkörper and exocytosis in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescent proteins (FPs) are powerful tools to investigate intracellular dynamics and protein localization. Cytoplasmic expression of FPs in fungal pathogens allows greater insight into invasion strategies and the host-pathogen interaction. Detection of their fluorescent signal depends on the right combination of microscopic setup and signal brightness. Slow rates of photo-bleaching are pivotal for in vivo observation of FPs over longer periods of time. Here, we test green-fluorescent proteins, including Aequorea coerulescens GFP (AcGFP), enhanced GFP (eGFP) from Aequorea victoria and a novel Zymoseptoria tritici codon-optimized eGFP (ZtGFP), for their usage in conventional and laser-enhanced epi-fluorescence, and confocal laser-scanning microscopy. We show that eGFP, expressed cytoplasmically in Z. tritici, is significantly brighter and more photo-stable than AcGFP. The codon-optimized ZtGFP performed even better than eGFP, showing significantly slower bleaching and a 20-30% further increase in signal intensity. Heterologous expression of all GFP variants did not affect pathogenicity of Z. tritici. Our data establish ZtGFP as the GFP of choice to investigate intracellular protein dynamics in Z. tritici, but also infection stages of this wheat pathogen inside host tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.