ArticleFungal genetics and biology : FG & B2015
A gene locus for targeted ectopic gene integration in Zymoseptoria tritici.
Article in Fungal genetics and biology : FG & B, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Life on a leaf: the epiphyte to pathogen continuum and interplay in the phyllosphere.BMC biology · 2024Review
- Multi-site fungicides suppress banana Panama disease, caused by Fusarium oxysporum f. sp. cubense Tropical Race 4.PLoS pathogens · 2022Article
- Zymoseptoria tritici white-collar complex integrates light, temperature and plant cues to initiate dimorphism and pathogenesis.Nature communications · 2022Article
- Article
- The identification of a transposon affecting the asexual reproduction of the wheat pathogen Zymoseptoria tritici.Molecular plant pathology · 2021Article
- Conditional promoters to investigate gene function during wheat infection by Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2021Article
- Asynchronous development of Zymoseptoria tritici infection in wheat.Fungal genetics and biology : FG & B · 2021Article
- Article
- A Novel Site-Specific Integration System for Genetic Modification ofG3 (Bethesda, Md.) · 2020Article
- CRISPR/Cas9-mediated endogenous gene tagging in Fusarium oxysporum.Fungal genetics and biology : FG & B · 2019Article
- Phosphopantetheinyl transferase (Ppt)-mediated biosynthesis of lysine, but not siderophores or DHN melanin, is required for virulence of Zymoseptoria tritici on wheat.Scientific reports · 2018Article
- Transposable element insertions shape gene regulation and melanin production in a fungal pathogen of wheat.BMC biology · 2018Article
- ATP prevents Woronin bodies from sealing septal pores in unwounded cells of the fungus Zymoseptoria tritici.Cellular microbiology · 2017Article
- A role for random, humidity-dependent epiphytic growth prior to invasion of wheat by Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2017Article
- Fluorescent markers of various organelles in the wheat pathogen Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2017Article
- Emerging Fungal Threats to Plants and Animals Challenge Agriculture and Ecosystem Resilience.Microbiology spectrum · 2017Review
- Development of a novel strategy for fungal transformation based on a mutant locus conferring carboxin-resistance in Magnaporthe oryzae.AMB Express · 2016Article
- Fluorescent markers of the endocytic pathway in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
- Red fluorescent proteins for imaging Zymoseptoria tritici during invasion of wheat.Fungal genetics and biology : FG & B · 2015Article
- A codon-optimized green fluorescent protein for live cell imaging in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
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Authors and funding
9 authors.
Funding
Abstract
Understanding the cellular organization and biology of fungal pathogens requires accurate methods for genomic integration of mutant alleles or fluorescent fusion-protein constructs. In Zymoseptoria tritici, this can be achieved by integrating of plasmid DNA randomly into the genome of this wheat pathogen. However, untargeted ectopic integration carries the risk of unwanted side effects, such as altered gene expression, due to targeting regulatory elements, or gene disruption following integration into protein-coding regions of the genome. Here, we establish the succinate dehydrogenase (sdi1) locus as a single "soft-landing" site for targeted ectopic integration of genetic constructs by using a carboxin-resistant sdi1(R) allele, carrying the point-mutation H267L. We use various green and red fluorescent fusion constructs and show that 97% of all transformants integrate correctly into the sdi1 locus as single copies. We also demonstrate that such integration does not affect the pathogenicity of Z. tritici, and thus the sdi1 locus is a useful tool for virulence analysis in genetically modified Z. tritici strains. Furthermore, we have developed a vector which facilitates yeast recombination cloning and thus allows assembly of multiple overlapping DNA fragments in a single cloning step for high throughput vector and strain generation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.