ArticleFungal genetics and biology : FG & B2015
Construction and high-throughput phenotypic screening ofZymoseptoria tritici over-expression strains.
Article in Fungal genetics and biology : FG & B, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Conditional promoters to investigate gene function during wheat infection by Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2021Article
- Tackling microbial threats in agriculture with integrative imaging and computational approaches.Computational and structural biotechnology journal · 2021Review
- A silver bullet in a golden age of functional genomics: the impact ofFungal biology and biotechnology · 2017Review
- Taming a wild beast: Developing molecular tools and new methods to understand the biology of Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Targeted gene deletion has been instrumental in elucidating many aspects of Zymoseptoria tritici pathogenicity. Gene over-expression is a complementary approach that is amenable to rapid strain construction and high-throughput screening, which has not been exploited to analyze Z. tritici, largely due to a lack of available techniques. Here we exploit the Gateway® cloning technology for rapid construction of over-expression vectors and improved homologous integration efficiency of a Z. tritici Δku70 strain to build a pilot over-expression library encompassing 32 genes encoding putative DNA binding proteins, GTPases or kinases. We developed a protocol using a Rotor-HDA robot for rapid and reproducible cell pinning for high-throughput in vitro screening. This screen identified an over-expression strain that demonstrated a marked reduction in hyphal production relative to the isogenic progenitor. This study provides a protocol for rapid generation of Z. tritici over-expression libraries and a technique for functional genomic screening in this important pathogen.
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Registered trials
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