ArticleFungal genetics and biology : FG & B2015
Exploitation of sulfonylurea resistance marker and non-homologous end joining mutants for functional analysis in Zymoseptoria tritici.
Article in Fungal genetics and biology : FG & B, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
- Biosynthesis of the Paecilomyces marquandii conidial pigment saintopin.Fungal biology and biotechnology · 2025Article
- Comparison of the impact of two key fungal signalling pathways on Zymoseptoria tritici infection reveals divergent contribution to invasive growth through distinct regulation of infection-associated genes.Molecular plant pathology · 2023Article
- The role of vegetative cell fusions in the development and asexual reproduction of the wheat fungal pathogen Zymoseptoria tritici.BMC biology · 2020Article
- Vib-PT, an Aromatic Prenyltransferase Involved in the Biosynthesis of Vibralactone fromApplied and environmental microbiology · 2020Article
- Overexpression ofMicroorganisms · 2019Article
- Fungal attack and host defence pathways unveiled in near-avirulent interactions of Penicillium expansum creA mutants on apples.Molecular plant pathology · 2018Article
- Four Inducible Promoters for Controlled Gene Expression in the Oleaginous YeastFrontiers in microbiology · 2016Article
- Candida glabrata: new tools and technologies-expanding the toolkit.FEMS yeast research · 2015Review
- Taming a wild beast: Developing molecular tools and new methods to understand the biology of Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
- Construction and high-throughput phenotypic screening ofZymoseptoria tritici over-expression strains.Fungal genetics and biology : FG & B · 2015Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The lack of techniques for rapid assembly of gene deletion vectors, paucity of selectable marker genes available for genetic manipulation and low frequency of homologous recombination are major constraints in construction of gene deletion mutants in Zymoseptoria tritici. To address these issues, we have constructed ternary vectors for Agrobacterium tumefaciens mediated transformation of Z. tritici, which enable the single step assembly of multiple fragments via yeast recombinational cloning. The sulfonylurea resistance gene, which is a mutated allele of the Magnaporthe oryzae ILV2 gene, was established as a new dominant selectable marker for Z. tritici. To increase the frequency of homologous recombination, we have constructed Z. tritici strains deficient in the non-homologous end joining pathway of DNA double stranded break repair by inactivating the KU70 and KU80 genes. Targeted gene deletion frequency increased to more than 85% in both Z. tritici ku70 and ku80 null strains, compared to ⩽10% seen in the wild type parental strain IPO323. The in vitro growth and in planta pathogenicity of the Z. tritici ku70 and ku80 null strains were comparable to strain IPO323. Together these molecular tools add significantly to the platform available for genomic analysis through targeted gene deletion or promoter replacements and will facilitate large-scale functional characterization projects in Z. tritici.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.