ArticleFungal genetics and biology : FG & B2015
Yeast recombination-based cloning as an efficient way of constructing vectors for Zymoseptoria tritici.
Article in Fungal genetics and biology : FG & B, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
- Functional characterization of extracellular and intracellular catalase-peroxidases involved in virulence of the fungal wheat pathogen Zymoseptoria tritici.Molecular plant pathology · 2024Article
- Virulent strains of Zymoseptoria tritici suppress the host immune response and facilitate the success of avirulent strains in mixed infections.PLoS pathogens · 2023Article
- Multi-site fungicides suppress banana Panama disease, caused by Fusarium oxysporum f. sp. cubense Tropical Race 4.PLoS pathogens · 2022Article
- Zymoseptoria tritici white-collar complex integrates light, temperature and plant cues to initiate dimorphism and pathogenesis.Nature communications · 2022Article
- Conditional promoters to investigate gene function during wheat infection by Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2021Article
- Genomic integration of unclonable gene expression cassettes in Saccharomyces cerevisiae using rapid cloning-free workflows.MicrobiologyOpen · 2020Article
- Class V chitin synthase and β(1,3)-glucan synthase co-travel in the same vesicle in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2020Article
- Phosphopantetheinyl transferase (Ppt)-mediated biosynthesis of lysine, but not siderophores or DHN melanin, is required for virulence of Zymoseptoria tritici on wheat.Scientific reports · 2018Article
- ATP prevents Woronin bodies from sealing septal pores in unwounded cells of the fungus Zymoseptoria tritici.Cellular microbiology · 2017Article
- Fluorescent markers of various organelles in the wheat pathogen Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2017Article
- A silver bullet in a golden age of functional genomics: the impact ofFungal biology and biotechnology · 2017Review
- Co-delivery of cell-wall-forming enzymes in the same vesicle for coordinated fungal cell wall formation.Nature microbiology · 2016Article
- Active diffusion and microtubule-based transport oppose myosin forces to position organelles in cells.Nature communications · 2016Article
- Four Inducible Promoters for Controlled Gene Expression in the Oleaginous YeastFrontiers in microbiology · 2016Article
- Construction of adenovirus vectors encoding the lumican gene by gateway recombinant cloning technology.International journal of ophthalmology · 2016Article
- Fluorescent markers of the microtubule cytoskeleton in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
- Conditional promoters for analysis of essential genes in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
- Fluorescent markers for the Spitzenkörper and exocytosis in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
- Fluorescent markers of the endocytic pathway in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
- Red fluorescent proteins for imaging Zymoseptoria tritici during invasion of wheat.Fungal genetics and biology : FG & B · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Many pathogenic fungi are genetically tractable. Analysis of their cellular organization and invasion mechanisms underpinning virulence determinants profits from exploiting such molecular tools as fluorescent fusion proteins or conditional mutant protein alleles. Generation of these tools requires efficient cloning methods, as vector construction is often a rate-limiting step. Here, we introduce an efficient yeast recombination-based cloning (YRBC) method to construct vectors for the fungus Zymoseptoria tritici. This method is of low cost and avoids dependency on the availability of restriction enzyme sites in the DNA sequence, as needed in more conventional restriction/ligation-based cloning procedures. Furthermore, YRBC avoids modification of the DNA of interest, indeed this potential risk limits the use of site-specific recombination systems, such as Gateway cloning. Instead, in YRBC, multiple DNA fragments, with 30bp overlap sequences, are transformed into Saccharomyces cerevisiae, whereupon homologous recombination generates the vector in a single step. Here, we provide a detailed experimental protocol and four vectors, each encoding a different dominant selectable marker cassette, that enable YRBC of constructs to be used in the wheat pathogen Z. tritici.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.