ArticleNucleic acids research2015
Ionic switch controls the DNA state in phage λ.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Viral Genome Delivery Across Bacterial Cell Surfaces.Annual review of microbiology · 2024Review
- Temperature-dependent ejection evolution arising from active and passive effects in DNA viruses.Biophysical journal · 2024Article
- Coinfecting phages impede each other's entry into the cell.Current biology : CB · 2024Article
- Temperature-induced DNA density transition in phage λ capsid revealed with contrast-matching SANS.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Geochemical constraints on bacteriophage infectivity in terrestrial environments.ISME communications · 2023Article
- AT-specific DNA visualization revisits the directionality of bacteriophage λ DNA ejection.Nucleic acids research · 2023Article
- Ion-dependent DNA configuration in bacteriophage capsids.Biophysical journal · 2021Article
- Article
- Influence of Microscopic Interactions on the Flexible Mechanical Properties of Viral DNA.Biophysical journal · 2018Article
- Temperature and pH dependence of DNA ejection from archaeal lemon-shaped virus His1.European biophysics journal : EBJ · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have recently found that DNA packaged in phage λ undergoes a disordering transition triggered by temperature, which results in increased genome mobility. This solid-to-fluid like DNA transition markedly increases the number of infectious λ particles facilitating infection. However, the structural transition strongly depends on temperature and ionic conditions in the surrounding medium. Using titration microcalorimetry combined with solution X-ray scattering, we mapped both energetic and structural changes associated with transition of the encapsidated λ-DNA. Packaged DNA needs to reach a critical stress level in order for transition to occur. We varied the stress on DNA in the capsid by changing the temperature, packaged DNA length and ionic conditions. We found striking evidence that the intracapsid DNA transition is 'switched on' at the ionic conditions mimicking those in vivo and also at the physiologic temperature of infection at 37°C. This ion regulated on-off switch of packaged DNA mobility in turn affects viral replication. These results suggest a remarkable adaptation of phage λ to the environment of its host bacteria in the human gut. The metastable DNA state in the capsid provides a new paradigm for the physical evolution of viruses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.