ArticleApplied and environmental microbiology2015
Flocculation of Escherichia coli Cells in Association with Enhanced Production of Outer Membrane Vesicles.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 26 citations in OpenAlex.
- De Novo Engineered Living Materials via Elastin-Like Polypeptide-Mediated Self-Assembly.ACS synthetic biology · 2026Article
- Molecular self-assembly mediates the flocculation activity of benzimidazole derivatives against E. coli.Scientific reports · 2025Article
- Airborne Cyanobacterial Toxins and Their Links to Neurodegenerative Diseases.Molecules (Basel, Switzerland) · 2025Review
- LuxR402 ofFrontiers in microbiology · 2023Article
- Cellulose-mediated floc formation by the activated sludge bacterium Shinella zoogloeoides ATCC 19623.BMC microbiology · 2022Article
- Incorporation ofFrontiers in microbiology · 2021Article
- Inducible asymmetric cell division and cell differentiation in a bacterium.Nature chemical biology · 2019Article
- Inducing flocculation of non-floc-forming Escherichia coli cells.World journal of microbiology & biotechnology · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial flocculation is a phenomenon of aggregation of dispersed bacterial cells in the form of flocs or flakes. In this study, the mechanism of spontaneous flocculation of Escherichia coli cells by overexpression of the bcsB gene was investigated. The flocculation induced by overexpression of bcsB was consistent among the various E. coli strains examined, including the K-12, B, and O strains, with flocs that resembled paper scraps in structure being about 1 to 2 mm. The distribution of green fluorescent protein-labeled E. coli cells within the floc structure was investigated by three-dimensional confocal laser scanning microscopy. Flocs were sensitive to proteinase K, indicating that the main component of the flocs was proteinous. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and nano-liquid chromatography tandem mass spectrometry analyses of the flocs strongly suggested the involvement of outer membrane vesicles (OMVs) in E. coli flocculation. The involvement of OMVs in flocculation was supported by transmission electron microscopy observation of flocs. Furthermore, bcsB-induced E. coli flocculation was greatly suppressed in strains with hypovesiculation phenotypes (ΔdsbA and ΔdsbB strains). Thus, our results demonstrate the strong correlation between spontaneous flocculation and enhanced OMV production of E. coli cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.