ArticleApplied and environmental microbiology2015
Development and Evaluation of Three Real-Time PCR Assays for Genotyping and Source Tracking Cryptosporidium spp. in Water.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 46 citations in OpenAlex.
- Zoonotic Transmission Assessment of Cryptosporidium spp. in Close Human-Pet Environments in Yunnan Province, China.Veterinary medicine and science · 2026Article
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- Can Wastewater Surveillance Enhance Genomic Tracking of Climate-Driven Pathogens?Microorganisms · 2025Review
- Stage-specific expression and divergent functions of two insulinase-like proteases associated with host infectivity in Cryptosporidium.PLoS neglected tropical diseases · 2025Article
- High prevalence and pathogenicity ofCurrent research in parasitology & vector-borne diseases · 2025Article
- A cholangiocyte organoid system forhLife · 2024Article
- Cryptosporidium parvum disrupts intestinal epithelial barrier in neonatal mice through downregulation of cell junction molecules.PLoS neglected tropical diseases · 2024Article
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- High zoonotic potential and heavy environmental burden ofOne health (Amsterdam, Netherlands) · 2023Article
- High infectivity and unique genomic sequence characteristics of Cryptosporidium parvum in China.PLoS neglected tropical diseases · 2022Article
- Detection ofMicroorganisms · 2022Review
- Molecular Diagnostic Tools Applied for Assessing Microbial Water Quality.International journal of environmental research and public health · 2022Review
- Standardization of molecular techniques for the detection and characterization of intestinal protozoa and other pathogens in humans.The journal of venomous animals and toxins including tropical diseases · 2022Article
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- Molecular identification ofInternational journal for parasitology. Parasites and wildlife · 2020Article
- Common occurrence of divergent Cryptosporidium species and Cryptosporidium parvum subtypes in farmed bamboo rats (Rhizomys sinensis).Parasites & vectors · 2020Article
- Cryptosporidium parvum and Cryptosporidium hominis subtypes in crab-eating macaques.Parasites & vectors · 2019Article
- Molecular detection and genotyping of pathogenic protozoan parasites in raw and treated water samples from southwest Colombia.Parasites & vectors · 2018Article
- Practical Guidance for Clinical Microbiology Laboratories: Laboratory Diagnosis of Parasites from the Gastrointestinal TractClinical microbiology reviews · 2018Review
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The occurrence of Cryptosporidium oocysts in drinking source water can present a serious public health risk. To rapidly and effectively assess the source and human-infective potential of Cryptosporidium oocysts in water, sensitive detection and correct identification of oocysts to the species level (genotyping) are essential. In this study, we developed three real-time PCR genotyping assays, two targeting the small-subunit (SSU) rRNA gene (18S-LC1 and 18S-LC2 assays) and one targeting the 90-kDa heat shock protein (hsp90) gene (hsp90 assay), and evaluated the sensitivity and Cryptosporidium species detection range of these assays. Using fluorescence resonance energy transfer probes and melt curve analysis, the 18S-LC1 and hsp90 assays could differentiate common human-pathogenic species (C. parvum, C. hominis, and C. meleagridis), while the 18S-LC2 assay was able to differentiate nonpathogenic species (such as C. andersoni) from human-pathogenic ones commonly found in source water. In sensitivity evaluations, the 18S-LC2 and hsp90 genotyping assays could detect as few as 1 Cryptosporidium oocyst per sample. Thus, the 18S-LC2 and hsp90 genotyping assays might be used in environmental monitoring, whereas the 18S-LC1 genotyping assay could be useful for genotyping Cryptosporidium spp. in clinical specimens or wastewater samples.
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