ArticleMolecular & cellular proteomics : MCP2015
Quantitative Circadian Phosphoproteomic Analysis of Arabidopsis Reveals Extensive Clock Control of Key Components in Physiological, Metabolic, and Signaling Pathways.
Article in Molecular & cellular proteomics : MCP, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
50 citing papers in PubMed, 105 citations in OpenAlex.
- Drought Stress Mediated Changes in Food Crops: Mechanisms and Remediation Strategies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Temperature compensation of the Arabidopsis circadian clock.Npj biological timing and sleep · 2025Review
- A Compensated Clock: Temperature and Nutritional Compensation Mechanisms Across Circadian Systems.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Circadian gating: concepts, processes, and opportunities.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- 40S Ribosomal protein S6 kinase integrates daylength perception and growth regulation in Arabidopsis thaliana.Plant physiology · 2024Article
- A phosphorylation-deficient ribosomal protein eS6 is largely functional inPlant direct · 2024Article
- Multi-omics landscape to decrypt the distinct flavonoid biosynthesis ofHorticulture research · 2024Article
- Circadian regulation of metabolism across photosynthetic organisms.The Plant journal : for cell and molecular biology · 2023Review
- A phospho-dawn of protein modification anticipates light onset in the picoeukaryote Ostreococcus tauri.Journal of experimental botany · 2023Article
- Genome-wide circadian gating of a cold temperature response in bread wheat.PLoS genetics · 2023Article
- The Case for the Target of Rapamycin Pathway as a Candidate Circadian Oscillator.International journal of molecular sciences · 2023Review
- Phytochromes and Their Role in Diurnal Variations of ROS Metabolism and Plant Proteome.International journal of molecular sciences · 2022Article
- Multi-omic analysis shows REVEILLE clock genes are involved in carbohydrate metabolism and proteasome function.Plant physiology · 2022Article
- Trihelix transcription factors GTL1 and DF1 prevent aberrant root hair formation in an excess nutrient condition.The New phytologist · 2022Article
- The intersection between circadian and heat-responsive regulatory networks controls plant responses to increasing temperatures.Biochemical Society transactions · 2022Review
- Phosphorylation of MdERF17 by MdMPK4 promotes apple fruit peel degreening during light/dark transitions.The Plant cell · 2022Article
- The enigmatic clock of dinoflagellates, is it unique?Frontiers in microbiology · 2022Review
- Hormonal and environmental signaling pathways target membrane water transport.Plant physiology · 2021Review
- The Arabidopsis Rho of Plants GTPase ROP1 Is a Potential Calcium-Dependent Protein Kinase (CDPK) Substrate.Plants (Basel, Switzerland) · 2021Article
- Diurnal dynamics of the Arabidopsis rosette proteome and phosphoproteome.Plant, cell & environment · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 4 countries.
Funding
Abstract
The circadian clock provides adaptive advantages to an organism, resulting in increased fitness and survival. The phosphorylation events that regulate circadian-dependent signaling and the processes which post-translationally respond to clock-gated signals are largely unknown. To better elucidate post-translational events tied to the circadian system we carried out a survey of circadian-regulated protein phosphorylation events in Arabidopsis seedlings. A large-scale mass spectrometry-based quantitative phosphoproteomics approach employing TiO2-based phosphopeptide enrichment techniques identified and quantified 1586 phosphopeptides on 1080 protein groups. A total of 102 phosphopeptides displayed significant changes in abundance, enabling the identification of specific patterns of response to circadian rhythms. Our approach was sensitive enough to quantitate oscillations in the phosphorylation of low abundance clock proteins (early flowering4; ELF4 and pseudoresponse regulator3; PRR3) as well as other transcription factors and kinases. During constant light, extensive cyclic changes in phosphorylation status occurred in critical regulators, implicating direct or indirect regulation by the circadian system. These included proteins influencing transcriptional regulation, translation, metabolism, stress and phytohormones-mediated responses. We validated our analysis using the elf4-211 allele, in which an S45L transition removes the phosphorylation herein identified. We show that removal of this phosphorylatable site diminishes interaction with early flowering3 (ELF3), a key partner in a tripartite evening complex required for circadian cycling. elf4-211 lengthens period, which increases with increasing temperature, relative to the wild type, resulting in a more stable temperature compensation of circadian period over a wider temperature range.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.