ArticleOncotarget2015
In vivo selection for spine-derived highly metastatic lung cancer cells is associated with increased migration, inflammation and decreased adhesion.
Article in Oncotarget, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Establishment and comprehensive characterization of a subline with highly bone-metastatic propensity derived from the lung adenocarcinoma A549 cell line.Journal of bone oncology · 2025Article
- Targeting ESM-1 inhibits spinal metastasis by disrupting tumor-endothelial adhesion.Molecular biomedicine · 2025Article
- GATA3-Driven ceRNA Network in Lung Adenocarcinoma Bone Metastasis Progression and Therapeutic Implications.Cancers · 2025Article
- Tumor treating fields suppress tumor cell growth and neurologic decline in models of spinal metastases.JCI insight · 2024Article
- Research progress in myosin light chain 9 in malignant tumors.Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2021Article
- How to Target Spinal Metastasis in Experimental Research: An Overview of Currently Used Experimental Mouse Models and Future Prospects.International journal of molecular sciences · 2021Review
- Proteomic analysis of lung cancer cells reveals a critical role of BCAT1 in cancer cell metastasis.Theranostics · 2021Article
- Identification of Cysteine Protease Inhibitor CST2 as a Potential Biomarker for Colorectal Cancer.Journal of Cancer · 2021Article
- Article
- Characterization of a Novel Murine Colon Carcinoma Subline with High-Metastatic Activity Established by In Vivo Selection Method.International journal of molecular sciences · 2020Article
- Human Platelet Lysates-Based Hydrogels: A Novel Personalized 3D Platform for Spheroid Invasion Assessment.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- HOXC10 overexpression promotes cell proliferation and migration in gastric cancer.Oncology reports · 2019Article
- Inhibition of yes-associated protein suppresses brain metastasis of human lung adenocarcinoma in a murine model.Journal of cellular and molecular medicine · 2018Article
- TIMP-1 downregulation modulates miR-125a-5p expression and triggers the apoptotic pathway.Oncotarget · 2018Article
- Gene expression profile analysis of the bone microenvironment in patients with spinal metastases.Oncology letters · 2018Article
- KOncotarget · 2017Article
- Persistent Exposure toFrontiers in cellular and infection microbiology · 2017Article
- Establishment of highly metastatic KRAS mutant lung cancer cell sublines in long-term three-dimensional low attachment cultures.PloS one · 2017Article
- MicroRNA-93-5p may participate in the formation of morphine tolerance in bone cancer pain mouse model by targeting Smad5.Oncotarget · 2016Article
- TQ inhibits hepatocellular carcinoma growth in vitro and in vivo via repression of Notch signaling.Oncotarget · 2015Article
Corrections and comments
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Authors and funding
19 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a murine spine metastasis model by screening five metastatic non-small cell lung cancer cell lines (PC-9, A549, NCI-H1299, NCI-H460, H2030). A549 cells displayed the highest tendency towards spine metastases. After three rounds of selection in vivo, we isolated a clone named A549L6, which induced spine metastasis in 80% of injected mice. The parameters of the A549L6 cell spinal metastatic mouse models were consistent with clinical spine metastasis features. All the spinal metastatic mice developed symptoms of nerve compression after 40 days. A549L6 cells had increased migration, invasiveness and decreased adhesion compared to the original A549L0 cells. In contrast, there was no significant differences in cell proliferation, apoptosis and sensitivity to chemotherapeutic agents such as cisplatin. Comparative transcriptomic analysis and real-time PCR analysis showed that expression of signaling molecules regulating several tumor properties including migration (MYL9), metastasis (CEACAM6, VEGFC, CX3CL1, CST1, CCL5, S100A9, IGF1, NOTCH3), adhesion (FN1, CEACAM1) and inflammation (TRAF2, NFκB2 and RelB) were altered in A549L6 cells. We suggest that migration, adhesion and inflammation related genes contribute to spine metastatic capacity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.