ArticlePloS one2015
Temperature and Development Impacts on Housekeeping Gene Expression in Cowpea Aphid, Aphis craccivora (Hemiptera: Aphidiae).
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
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- Reference Genes for Expression Analyses by qRT-PCR inBiology · 2025Article
- Article
- Comparative transcriptomics of a generalist aphid,Frontiers in plant science · 2024Article
- Insecticidal Effect of the Entomopathogenic FungusInsects · 2023Article
- Transposon accumulation at xenobiotic gene family loci in aphids.Genome research · 2023Article
- Identification and Validation of Reference Genes for Expression Analysis Using qRT-PCR inInsects · 2022Article
- Reference Genes for Expression Analyses by qRT-PCR inInsects · 2022Article
- Selection and validation of experimental condition-specific reference genes for qRT-PCR in Metopolophium dirhodum (Walker) (Hemiptera: Aphididae).Scientific reports · 2020Article
- Comprehensive Assessment of Candidate Reference Genes for Gene Expression Studies Using RT-qPCR inGenes · 2020Article
- Transcriptional changes in the aphid species Myzus cerasi under different host and environmental conditions.Insect molecular biology · 2020Article
- Identification and Characterization of Neuropeptides and Their G Protein-Coupled Receptors (GPCRs) in the Cowpea AphidFrontiers in endocrinology · 2020Article
- Function Analysis of P450 and GST Genes to Imidacloprid inFrontiers in physiology · 2020Article
- Identification of sources of resistance in cowpea mini core accessions toEuphytica: Netherlands journal of plant breeding · 2020Article
- Identification and Validation of Reference Genes for Quantitative Gene Expression Analysis inFrontiers in physiology · 2020Article
- Selection of Reference Genes for RT-qPCR Analysis Under Extrinsic Conditions in the Hawthorn Spider Mite,Frontiers in physiology · 2020Article
- Selection of Reference Genes for RT-qPCR Analysis Under Intrinsic Conditions in the Hawthorn Spider Mite,Frontiers in physiology · 2019Article
- Selection and Validation of Reference Genes for RT-qPCR Analysis of the Ladybird BeetleFrontiers in physiology · 2018Article
- Selection of appropriate reference genes for RT-qPCR analysis in Propylea japonica (Coleoptera: Coccinellidae).PloS one · 2018Article
- Selection of Reference Genes for the Normalization of RT-qPCR Data in Gene Expression Studies in Insects: A Systematic Review.Frontiers in physiology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative real-time PCR (qRT-PCR) is a powerful technique to quantify gene expression. To standardize gene expression studies and obtain more accurate qRT-PCR analysis, normalization relative to consistently expressed housekeeping genes (HKGs) is required. In this study, ten candidate HKGs including elongation factor 1 α (EF1A), ribosomal protein L11 (RPL11), ribosomal protein L14 (RPL14), ribosomal protein S8 (RPS8), ribosomal protein S23 (RPS23), NADH-ubiquinone oxidoreductase (NADH), vacuolar-type H+-ATPase (ATPase), heat shock protein 70 (HSP70), 18S ribosomal RNA (18S), and 12S ribosomal RNA (12S) from the cowpea aphid, Aphis craccivora Koch were selected. Four algorithms, geNorm, Normfinder, BestKeeper, and the ΔCt method were employed to evaluate the expression profiles of these HKGs as endogenous controls across different developmental stages and temperature regimes. Based on RefFinder, which integrates all four analytical algorithms to compare and rank the candidate HKGs, RPS8, RPL14, and RPL11 were the three most stable HKGs across different developmental stages and temperature conditions. This study is the first step to establish a standardized qRT-PCR analysis in A. craccivora following the MIQE guideline. Results from this study lay a foundation for the genomics and functional genomics research in this sap-sucking insect pest with substantial economic impact.
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