Evidence map›Paper›PMID 26090087›Full record

ArticleJournal of tissue engineering2015

Analysis of type II diabetes mellitus adipose-derived stem cells for tissue engineering applications.

Danielle Marie Minteer, Matthew T Young, Yen-Chih Lin, Patrick J Over, J Peter Rubin, Jorg C Gerlach, Kacey G Marra

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of tissue engineering, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Danielle Marie MinteerDepartment of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.
Matthew T YoungMcGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Yen-Chih LinMcGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA ; Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA.
Patrick J OverMcGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
J Peter RubinDepartment of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA ; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA ; Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA.
Jorg C GerlachDepartment of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA ; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA ; Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA.
Kacey G MarraDepartment of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA ; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA ; Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA.

Funding

3D Culture of Adipose Tissue for Screening Obesity-related DrugsR01DK089190 · NIDDK · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI MARRA, KACEY G · 2010 to 2012
$988k
NIDDK NIH HHS R01 DK089190
6 · The paper itself

Abstract

To address the functionality of diabetic adipose-derived stem cells in tissue engineering applications, adipose-derived stem cells isolated from patients with and without type II diabetes mellitus were cultured in bioreactor culture systems. The adipose-derived stem cells were differentiated into adipocytes and maintained as functional adipocytes. The bioreactor system utilizes a hollow fiber-based technology for three-dimensional perfusion of tissues in vitro, creating a model in which long-term culture of adipocytes is feasible, and providing a potential tool useful for drug discovery. Daily metabolic activity of the adipose-derived stem cells was analyzed within the medium recirculating throughout the bioreactor system. At experiment termination, tissues were extracted from bioreactors for immunohistological analyses in addition to gene and protein expression. Type II diabetic adipose-derived stem cells did not exhibit significantly different glucose consumption compared to adipose-derived stem cells from patients without type II diabetes (p > 0.05, N = 3). Expression of mature adipocyte genes was not significantly different between diabetic/non-diabetic groups (p > 0.05, N = 3). Protein expression of adipose tissue grown within all bioreactors was verified by Western blotting.The results from this small-scale study reveal adipose-derived stem cells from patients with type II diabetes when removed from diabetic environments behave metabolically similar to the same cells of non-diabetic patients when cultured in a three-dimensional perfusion bioreactor, suggesting that glucose transport across the adipocyte cell membrane, the hindrance of which being characteristic of type II diabetes, is dependent on environment. The presented observation describes a tissue-engineered tool for long-term cell culture and, following future adjustments to the culture environment and increased sample sizes, potentially for anti-diabetic drug testing.

Indexed as

Adipocytesadipogenesisbioreactorcell and tissue culturecell culturesdiabetes researchmesenchymal stem cellsstem cells—adiposethree-dimensionaltissue culture modelstissue engineering

Identifiers

PMID26090087
PMCPMC4456321

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.