ArticleJournal of assisted reproduction and genetics2015
Successful cryopreservation of whole sheep ovary by using DMSO-free cryoprotectant.
Article in Journal of assisted reproduction and genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Selective under-representation of transcripts encoding membrane-integrated architectures following neural tissue vitrification.Biochemistry and biophysics reports · 2026Article
- Vitrification and Rewarming of Magnetic Nanoparticle-Loaded Rat Hearts.Advanced materials technologies · 2022Article
- Cryopreservation of NK and T Cells Without DMSO for Adoptive Cell-Based Immunotherapy.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2021Review
- Whole Ovary Cryopreservation and Transplantation: A Systematic Review of Challenges and Research Developments in Animal Experiments and Humans.Journal of clinical medicine · 2020Review
- Umbilical cord tissue cryopreservation: a short review.Stem cell research & therapy · 2018Review
- Novel Approach Toward the Generation of Tissue Engineered Heart Valve by Using Combination of Antioxidant and Detergent: A Potential Therapy in Cardiovascular Tissue Engineering.Tissue engineering and regenerative medicine · 2017Article
- Complete protection against cryodamage of cryopreserved whole bovine and human ovaries using DMSO as a cryoprotectant.Journal of assisted reproduction and genetics · 2017Article
- Bioengineering Strategies to Treat Female Infertility.Tissue engineering. Part B, Reviews · 2017Article
- Synergistic Development of Biochips and Cell Preservation Methodologies: A Tale of Converging Technologies.Current stem cell reports · 2017Article
- Cold-inducible proteins CIRP and RBM3, a unique couple with activities far beyond the cold.Cellular and molecular life sciences : CMLS · 2016Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe study aims to assess the protective effects of dimethyl sulfoxide (DMSO)-free solution based on trehalose on the cryopreservation of a whole sheep ovary and evaluate its use as an efficient cryoprotectant.
methodTwenty-one ovaries collected from 6- to 8-month-old non-pregnant female sheep were randomly distributed into three groups, namely, a fresh group, a DMSO-free group, and a DMSO group. The morphology, cell apoptosis (by hematoxylin and eosin (HE) staining and terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling (TUNEL) assay), and mRNA transcript of Bcl-2-associated X protein (BAX) and cold inducible RNA-binding protein (CIRP) (by real-time PCR) of the thawed sheep ovaries and fresh controls were tested to establish a criterion for appraising the results of the cryopreservation.
results(i) The histological assessment indicated that the structure of the DMSO-free ovaries remained largely intact and comparable to those of the fresh control groups; whereas, significant damage was observed in the ovaries of the DMSO group (P < 0.05). (ii) The TUNEL assay and mRNA transcript of the BAX assessment showed that the apoptosis parameter in the fresh group was the lowest among all the groups (P < 0.05), and the parameter in the DMSO-free group was significantly lower than that in the DMSO group (P < 0.05). (iii) The level of the CIRP transcripts increased the most in the DMSO-free group followed by the DMSO group and the fresh control group (P < 0.05).
conclusionsThese results indicate that a DMSO-free cryoprotectant solution, especially a trehalose cryoprotectant, is an efficient cryoprotectant and has a beneficial effect on the cryopreservation of whole sheep ovaries.
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Registered trials
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