Trial reportExperimental biology and medicine (Maywood, N.J.)2015
Expansion and long-term culture of human spermatogonial stem cells via the activation of SMAD3 and AKT pathways.
Trial report in Experimental biology and medicine (Maywood, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 55 citations in OpenAlex.
- Comparison of reproductive performance and functional analysis of spermatogenesis factors between domestic yak and semi-wild blood yak.BMC genomics · 2025Article
- Epigenetic characterization of adult rhesus monkey spermatogonial stem cells identifies key regulators of stem cell homeostasis.Nucleic acids research · 2024Article
- Conserved Transcriptome Features Define Prepubertal Primate Spermatogonial Stem Cells as AInternational journal of molecular sciences · 2023Article
- Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Favorable culture conditions for spermatogonial propagation in human and non-human primate primary testicular cell cultures: a systematic review and meta-analysis.Frontiers in cell and developmental biology · 2023Article
- The PI3K/AKT signaling pathway: How does it regulate development of Sertoli cells and spermatogenic cells?Histology and histopathology · 2022Review
- Spermatogonial Stem Cell-Based Therapies: Taking Preclinical Research to the Next Level.Frontiers in endocrinology · 2022Review
- Review
- Review
- Metabolic Requirements for Spermatogonial Stem Cell Establishment and Maintenance In Vivo and In Vitro.International journal of molecular sciences · 2021Review
- Recent advances: fertility preservation and fertility restoration options for males and females.Faculty reviews · 2021Review
- ITGA6+ Human Testicular Cell Populations Acquire a Mesenchymal Rather than Germ Cell Transcriptional Signature during Long-Term Culture.International journal of molecular sciences · 2020Article
- Stem cell-based therapies for fertility preservation in males: Current status and future prospects.World journal of stem cells · 2020Review
- Hsa-miR-1908-3p Mediates the Self-Renewal and Apoptosis of Human Spermatogonial Stem Cells via Targeting KLF2.Molecular therapy. Nucleic acids · 2020Article
- Spermatogonial Stem Cell Culture in Oncofertility.The Urologic clinics of North America · 2020Review
- Spermatogonial Stem Cells for In Vitro Spermatogenesis and In Vivo Restoration of Fertility.Cells · 2020Review
- Xeno-Free Propagation of Spermatogonial Stem Cells from Infant Boys.International journal of molecular sciences · 2019Article
- Review
- Human Testis Extracellular Matrix Enhances Human Spermatogonial Stem Cell SurvivalTissue engineering. Part A · 2019Article
- Role of stem cells in fertility preservation: current insights.Stem cells and cloning : advances and applications · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spermatogonial stem cells (SSCs) can differentiate into spermatids, reflecting that they could be used in reproductive medicine for treating male infertility. SSCs are able to become embryonic stem-like cells with the potentials of differentiating into numerous cell types of the three germ layers and they can transdifferentiate to mature and functional cells of other lineages, highlighting significant applications of human SSCs for treating human diseases. However, human SSCs are very rare and a long-term culture system of human SSCs has not yet established. This aim of study was to isolate, identify and culture human SSCs for a long period. We isolated GPR125-positive spermatogonia with high purity and viability from adult human testicular tissues utilizing the two-step enzymatic digestion and magnetic-activated cell sorting with antibody against GPR125. These freshly isolated cells expressed a number of markers for SSCs, including GPR125, PLZF, GFRA1, RET, THY1, UCHL1 and MAGEA4, but not the hallmarks for spermatocytes and spermatozoa, e.g. SYCP1, SYCP3, PRM1, and TNP1. The isolated human SSCs could be cultured for two months with a significant increase of cell number with the defined medium containing growth factors and hydrogel. Notably, the expression of numerous SSC markers was maintained during the cultivation of human SSCs. Furthermore, SMAD3 and AKT phosphorylation was enhanced during the culture of human SSCs. Collectively, these results suggest that human SSCs can be cultivated for a long period and expanded whilst retaining an undifferentiated status via the activation of SMAD3 and AKT pathways. This study could provide sufficient cells of SSCs for their basic research and clinic applications in reproductive and regenerative medicine.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.