ArticleCroatian medical journal2015
Analysis of mixtures using next generation sequencing of mitochondrial DNA hypervariable regions.
Article in Croatian medical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Comparison of classic Sanger and next generation sequencing mitotypes of second world war victims from Konfin I mass grave.International journal of legal medicine · 2026Article
- Capillary-Electrophoresis-Based Species Barcoding of Big Cats: CR-mtDNA-Length Polymorphism.Life (Basel, Switzerland) · 2024Article
- Article
- Estimating individual mtDNA haplotypes in mixed DNA samples by combining MinION and MiSeq.International journal of legal medicine · 2022Article
- Developmental validation of the MGIEasy Signature Identification Library Prep Kit, an all-in-one multiplex system for forensic applications.International journal of legal medicine · 2021Article
- A Continuous Statistical Phasing Framework for the Analysis of Forensic Mitochondrial DNA Mixtures.Genes · 2021Article
- Estimation of the number of contributors to mixed samples of DNA by mitochondrial DNA analyses using massively parallel sequencing.International journal of legal medicine · 2020Article
- Nanopore sequencing: An enrichment-free alternative to mitochondrial DNA sequencing.Electrophoresis · 2019Article
- Detection of Innate and Artificial Mitochondrial DNA Heteroplasmy by Massively Parallel Sequencing: Considerations for Analysis.Journal of Korean medical science · 2018Article
- Massively parallel sequencing techniques for forensics: A review.Electrophoresis · 2018Review
- Massively parallel sequencing-enabled mixture analysis of mitochondrial DNA samples.International journal of legal medicine · 2018Article
- Assessment of mitochondrial DNA heteroplasmy detected on commercial panel using MPS system with artificial mixture samples.International journal of legal medicine · 2018Article
- Article
- MPS analysis of the mtDNA hypervariable regions on the MiSeq with improved enrichment.International journal of legal medicine · 2017Article
- Population genomics reveals that within-fungus polymorphism is common and maintained in populations of the mycorrhizal fungus Rhizophagus irregularis.The ISME journal · 2016Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo apply massively parallel and clonal sequencing (next generation sequencing or NGS) to the analysis of forensic mixed samples.
methodsA duplex polymerase chain reaction (PCR) assay targeting the mitochondrial DNA (mtDNA) hypervariable regions I/II (HVI/HVII) was developed for NGS analysis on the Roche 454 GS Junior instrument. Eight sets of multiplex identifier-tagged 454 fusion primers were used in a combinatorial approach for amplification and deep sequencing of up to 64 samples in parallel.
resultsThis assay was shown to be highly sensitive for sequencing limited DNA amounts (~100 mtDNA copies) and analyzing contrived and biological mixtures with low level variants (~1%) as well as "complex" mixtures (≥3 contributors). PCR artifact "hybrid" sequences generated by jumping PCR or template switching were observed at a low level (<2%) in the analysis of mixed samples but could be eliminated by reducing the PCR cycle number.
conclusionThis study demonstrates the power of NGS technologies targeting the mtDNA HVI/HVII regions for analysis of challenging forensic samples, such as mixtures and specimens with limited DNA.
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