ArticleScientific reports2015
Analysis of Wnt signalling dynamics during colon crypt development in 3D culture.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 15 citations in OpenAlex.
- Micro-cold-forming: a simple, rapid, and inexpensive method for the fabrication of microcavities for 3D cell culture.RSC advances · 2026Article
- CD11b/CD86 involved in the microenvironment of colorectal cancer by promoting Wnt signaling activation.Cancer medicine · 2024Article
- Patient-derived Colonoids From Disease-spared Tissue Retain Inflammatory Bowel Disease-specific Transcriptomic Signatures.Gastro hep advances · 2023Article
- Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine.Communications biology · 2021Article
- IRF2 maintains the stemness of colonic stem cells by limiting physiological stress from interferon.Scientific reports · 2020Article
- Loss of Bcl-G, a Bcl-2 family member, augments the development of inflammation-associated colorectal cancer.Cell death and differentiation · 2020Article
- Repurposing the selective estrogen receptor modulatorEMBO molecular medicine · 2019Article
- Review
- Vitamin D Regulation of the Uridine Phosphorylase 1 Gene and Uridine-Induced DNA Damage in Colon in African Americans and European Americans.Gastroenterology · 2018Article
- Cytoglobin affects tumorigenesis and the expression of ulcerative colitis-associated genes under chemically induced colitis in mice.Scientific reports · 2018Article
- Colon organoid formation and cryptogenesis are stimulated by growth factors secreted from myofibroblasts.PloS one · 2018Article
- Molecular mechanisms involved in the adaptive regulation of the colonic thiamin pyrophosphate uptake process.American journal of physiology. Cell physiology · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many systems biology studies lack context-relevant data and as a consequence the predictive capabilities can be limited in developing targeted cancer therapeutics. Production of colon crypt in vitro is ideal for studying colon systems biology. This report presents the first production of, to our knowledge, physiologically-shaped, functional colon crypts in vitro (i.e. single crypts with cells expressing Mucin 2 and Chromogranin A). Time-lapsed monitoring of crypt formation revealed an increased frequency of single-crypt formation in the absence of noggin. Using quantitative 3D immunofluorescence of β-catenin and E-cadherin, spatial-temporal dynamics of these proteins in normal colon crypt cells stimulated with Wnt3A or inhibited by cycloheximide has been measured. Colon adenoma cultures established from APC(min/+) mouse have developmental differences and β-catenin spatial localization compared to normal crypts. Quantitative data describing the effects of signalling pathways and proteins dynamics for both normal and adenomatous colon crypts is now within reach to inform a systems approach to colon crypt biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.