ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2015
Enhanced γ-H2AX DNA damage foci detection using multimagnification and extended depth of field in imaging flow cytometry.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- DNA Damage and Inflammatory Response of p53 Null H358 Non-Small Cell Lung Cancer Cells to X-Ray Exposure Under Chronic Hypoxia.International journal of molecular sciences · 2024Article
- Determination of dose-response calibration curves for gamma radiation using gamma-H2AX immunofluorescence based biodosimetry.Reports of practical oncology and radiotherapy : journal of Greatpoland Cancer Center in Poznan and Polish Society of Radiation Oncology · 2024Article
- Unraveling the association between major depressive disorder and senescent biomarkers in immune cells of older adults: a single-cell phenotypic analysis.Frontiers in aging · 2024Article
- The Imaging Flow Cytometry-Based Cytokinesis-Block MicroNucleus (CBMN) Assay.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Inter-laboratory automation of the in vitro micronucleus assay using imaging flow cytometry and deep learning.Archives of toxicology · 2021Article
- MicroRNA miR-24-3p reduces DNA damage responses, apoptosis, and susceptibility to chronic obstructive pulmonary disease.JCI insight · 2021Article
- TRIM29 is required for efficient recruitment of 53BP1 in response to DNA double-strand breaks in vertebrate cells.FEBS open bio · 2020Article
- EphA5 Silencing Increases the Radiosensitivity of ESCC Cells Through ATM-Dependent Pathway.Cancer management and research · 2020Article
- Development of a high-throughput γ-H2AX assay based on imaging flow cytometry.Radiation oncology (London, England) · 2019Article
- Label-free chemical imaging flow cytometry by high-speed multicolor stimulated Raman scattering.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Telomere elongation through hTERT immortalization leads to chromosome repositioning in control cells and genomic instability in Hutchinson-Gilford progeria syndrome fibroblasts, expressing a novel SUN1 isoform.Genes, chromosomes & cancer · 2019Article
- Knockdown of PKM2 enhances radiosensitivity of cervical cancer cells.Cancer cell international · 2019Article
- Automation of the in vitro micronucleus assay using the ImagestreamCytometry. Part A : the journal of the International Society for Analytical Cytology · 2018Article
- An open-source solution for advanced imaging flow cytometry data analysis using machine learning.Methods (San Diego, Calif.) · 2017Article
- The PARP-1 inhibitor Olaparib suppresses BRCA1 protein levels, increases apoptosis and causes radiation hypersensitivity in BRCA1Journal of Cancer · 2017Article
- Condensin II mutation causes T-cell lymphoma through tissue-specific genome instability.Genes & development · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accurate and rapid methods for the detection of DNA damage foci in eukaryotic cells are central to DNA repair studies, which identify differences in DNA repair capacity in cell lines. Such assays have been important in delineating mechanisms of DNA repair in human cells. Previously we were the first to demonstrate the use of imaging flow cytometry for the detection of γ-H2AX foci in cells exposed to ionizing radiation causing the induction of DNA strand breaks. In this report we extend these studies and show an enhancement of foci quantitation and image resolution using next generation imaging flow cytometry with the Amnis Imagestream(X) Mark II. We demonstrate using cell lines derived from normal individuals, and DNA double strand break repair defective cells that the number of foci observed is significantly increased when using 60× as compared to 40× magnification. Also, foci numbers and resolution is further increased with the application of the focus stacking (Extended Depth of Field-EDF) capacity activated. This report represents the first such demonstration of multimagnification and EDF for the enhanced quantitation of DNA damage in cells and provides a level of resolution, which near matches in situ microscopy methods for the detection of γ-H2AX foci.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.