ArticleAnalytical chemistry2015
Use of Charged Nanoparticles in NMR-Based Metabolomics for Spectral Simplification and Improved Metabolite Identification.
Article in Analytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- NMR spectral simplification with imidazolium ionic liquid-coated silica nanoparticles.Magnetic resonance letters · 2026Article
- Excited-state observation of active K-Ras reveals differential structural dynamics of wild-type versus oncogenic G12D and G12C mutants.Nature structural & molecular biology · 2023Article
- Biocorona on Iron Oxide Nanoparticles in a Complex Biotechnological Environment: Analysis of Proteins, Lipids, and Carbohydrates.Small science · 2023Article
- A simple and sensitive detection of the binding ligands by using the receptor aggregation and NMR spectroscopy: a test case of the maltose binding protein.Journal of biomolecular NMR · 2021Article
- NMR techniques in studying water in biotechnological systems.Biophysical reviews · 2020Review
- Functional protein dynamics on uncharted time scales detected by nanoparticle-assisted NMR spin relaxation.Science advances · 2019Article
- Nanoparticle-Assisted Metabolomics.Metabolites · 2018Review
- Integrative metabolomics as emerging tool to study autophagy regulation.Microbial cell (Graz, Austria) · 2017Review
- Knowns and unknowns in metabolomics identified by multidimensional NMR and hybrid MS/NMR methods.Current opinion in biotechnology · 2017Review
- Recent Advances in NMR-Based Metabolomics.Analytical chemistry · 2017Review
- Comprehensive Metabolite Identification Strategy Using Multiple Two-Dimensional NMR Spectra of a Complex Mixture Implemented in the COLMARm Web Server.Analytical chemistry · 2016Article
- Residue-Specific Interactions of an Intrinsically Disordered Protein with Silica Nanoparticles and their Quantitative Prediction.The journal of physical chemistry. C, Nanomaterials and interfaces · 2016Article
- Review
- Nanoparticle-Assisted Removal of Protein in Human Serum for Metabolomics Studies.Analytical chemistry · 2016Article
- Identification of the Metabolic Enzyme Involved Morusin Metabolism and Characterization of Its Metabolites by Ultraperformance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (UPLC/Q-TOF-MS/MS).Evidence-based complementary and alternative medicine : eCAM · 2016Article
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Authors and funding
5 authors.
Funding
Abstract
Metabolomics aims at a complete characterization of all metabolites in biological samples in terms of both their identities and concentrations. Because changes of metabolites and their concentrations are a direct reflection of cellular activity, it allows for a better understanding of cellular processes and function to be obtained. Although NMR spectroscopy is routinely applied to complex biological mixtures without purification, overlapping NMR peaks often pose a challenge for the comprehensive and accurate identification of the underlying metabolites. To address this problem, we present a novel nanoparticle-based strategy that differentiates between metabolites based on their electric charge. By adding electrically charged silica nanoparticles to the solution NMR sample, metabolites of opposite charge bind to the nanoparticles and their NMR signals are weakened or entirely suppressed due to peak broadening caused by the slow rotational tumbling of the nanometer-sized nanoparticles. Comparison of the edited with the original spectrum significantly facilitates analysis and reduces ambiguities in the identification of metabolites. This method makes NMR directly sensitive to the detection of molecular charges at constant pH, as demonstrated here both for model mixtures and human urine. The simplicity of the approach should make it useful for a wide range of metabolomics applications.
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