ArticleAnalytical chemistry2015
Definitive Screening Design Optimization of Mass Spectrometry Parameters for Sensitive Comparison of Filter and Solid Phase Extraction Purified, INLIGHT Plasma N-Glycans.
Article in Analytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
- N-Glycosylation modulators for targeted manipulation of glycosylation for monoclonal antibodies.Applied microbiology and biotechnology · 2025Article
- Recent Advances in Labeling-Based Quantitative Glycomics: From High-Throughput Quantification to Structural Elucidation.Proteomics · 2025Review
- Definitive Screening Designs to Optimize Library-Free DIA-MS Identification and Quantification of Neuropeptides.Journal of proteome research · 2023Article
- High sensitivity glycomics in biomedicine.Mass spectrometry reviews · 2022Review
- Utilizing liquid chromatography, ion mobility spectrometry, and mass spectrometry to assess INLIGHT™ derivatized N-linked glycans in biological samples.Analytical and bioanalytical chemistry · 2022Article
- In a pursuit of optimal glycan fluorescent label for negative MS mode for high-throughput N-glycan analysis.Frontiers in chemistry · 2022Article
- Multiplexed quantitative neuropeptidomics via DiLeu isobaric tagging.Methods in enzymology · 2022Article
- Developing mass spectrometry for the quantitative analysis of neuropeptides.Expert review of proteomics · 2021Review
- Mass Spectrometric Profiling of Neuropeptides in Response to Copper Toxicity via Isobaric Tagging.Chemical research in toxicology · 2021Article
- Enhanced protocol for quantitative N-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™.Analytical and bioanalytical chemistry · 2020Article
- NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.Molecular & cellular proteomics : MCP · 2020Article
- Multiplex Quantitative Glycomics Enabled by Periodate Oxidation and Triplex Mass Defect Isobaric Multiplex Reagents for Carbonyl-Containing Compound Tags.Analytical chemistry · 2019Article
- Towards a standardized bioinformatics infrastructure for N- and O-glycomics.Nature communications · 2019Review
- Seasonal Variations in the Metabolome and Bioactivity Profile ofMarine drugs · 2018Article
- Quantitative Glycomic Analysis by Mass-Defect-Based Dimethyl Pyrimidinyl Ornithine (DiPyrO) Tags and High-Resolution Mass Spectrometry.Analytical chemistry · 2018Article
- Targeted MultiNotch MSAnalytical chemistry · 2018Article
- Direct comparison of derivatization strategies for LC-MS/MS analysis of N-glycans.The Analyst · 2017Article
- Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides.Scientific reports · 2017Article
- Development of a hydrophilic interaction liquid chromatography coupled with matrix-assisted laser desorption/ionization-mass spectrometric imaging platform for N-glycan relative quantitation using stable-isotope labeled hydrazide reagents.Analytical and bioanalytical chemistry · 2017Article
- Recent advances in mass spectrometric analysis of glycoproteins.Electrophoresis · 2017Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
High-throughput, quantitative processing of N-linked glycans would facilitate large-scale studies correlating the glycome with disease and open the field to basic and applied researchers. We sought to meet these goals by coupling filter-aided-N-glycan separation (FANGS) to the individuality normalization when labeling with glycan hydrazide tags (INLIGHT) for analysis of plasma. A quantitative comparison of this method was conducted against solid phase extraction (SPE), a ubiquitous and trusted method for glycan purification. We demonstrate that FANGS-INLIGHT purification was not significantly different from SPE in terms of glycan abundances, variability, functional classes, or molecular weight distributions. Furthermore, to increase the depth of glycome coverage, we executed a definitive screening design of experiments (DOE) to optimize the MS parameters for glycan analyses. We optimized MS parameters across five N-glycan responses using a standard glycan mixture, translated these to plasma and achieved up to a 3-fold increase in ion abundances.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.