ArticlePloS one2015
Effect of X-Irradiation at Different Stages in the Cell Cycle on Individual Cell-Based Kinetics in an Asynchronous Cell Population.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
- Time-lapse imaging of cells in spatially fractionated X-ray fields using a mini beam as an alternative to accelerator-based sub-millimeter beams.Journal of radiation research · 2025Article
- DNA damage response in a 2D-culture model by diffusing alpha-emitters radiation therapy (Alpha-DaRT).Scientific reports · 2024Article
- A novel multifunctional radioprotective strategy using P7C3 as a countermeasure against ionizing radiation-induced bone loss.Bone research · 2023Article
- An updated view into the cell cycle kinetics of human T lymphocytes and the impact of irradiation.Scientific reports · 2022Article
- Article
- Alpelisib and radiotherapy treatment enhances Alisertib-mediated cervical cancer tumor killing.American journal of cancer research · 2021Article
- Fluctuation in radioresponse of HeLa cells during the cell cycle evaluated based on micronucleus frequency.Scientific reports · 2020Article
- Radiation-induced abnormal centrosome amplification and mitotic catastrophe in human cervical tumor HeLa cells and murine mammary tumor EMT6 cells.Journal of clinical biochemistry and nutrition · 2020Article
- Sample-based modeling reveals bidirectional interplay between cell cycle progression and extrinsic apoptosis.PLoS computational biology · 2020Article
- WEE1 inhibition enhances sensitivity to hypoxia/reoxygenation in HeLa cells.Journal of radiation research · 2019Article
- Cell Cycle Changes after Glioblastoma Stem Cell Irradiation: The Major Role of RAD51.International journal of molecular sciences · 2018Article
- Temporo-spatial cell-cycle kinetics in HeLa cells irradiated by Ir-192 high dose-rate remote afterloading system (HDR-RALS).Radiation oncology (London, England) · 2016Article
- Article
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7 authors.
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Abstract
Using an asynchronously growing cell population, we investigated how X-irradiation at different stages of the cell cycle influences individual cell-based kinetics. To visualize the cell-cycle phase, we employed the fluorescent ubiquitination-based cell cycle indicator (Fucci). After 5 Gy irradiation, HeLa cells no longer entered M phase in an order determined by their previous stage of the cell cycle, primarily because green phase (S and G2) was less prolonged in cells irradiated during the red phase (G1) than in those irradiated during the green phase. Furthermore, prolongation of the green phase in cells irradiated during the red phase gradually increased as the irradiation timing approached late G1 phase. The results revealed that endoreduplication rarely occurs in this cell line under the conditions we studied. We next established a method for classifying the green phase into early S, mid S, late S, and G2 phases at the time of irradiation, and then attempted to estimate the duration of G2 arrest based on certain assumptions. The value was the largest when cells were irradiated in mid or late S phase and the smallest when they were irradiated in G1 phase. In this study, by closely following individual cells irradiated at different cell-cycle phases, we revealed for the first time the unique cell-cycle kinetics in HeLa cells that follow irradiation.
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