ArticleInternational journal of ophthalmology2015
Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.
Article in International journal of ophthalmology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Role of microRNAs in immunoregulatory functions of epithelial cells.BMC immunology · 2024Review
- Host cell responses toFrontiers in cellular and infection microbiology · 2024Article
- Immune Checkpoint Inhibitor-Based Combination Therapy for Colorectal Cancer: An Overview.International journal of general medicine · 2023Review
- Characterization of fungal exposure and dectin-1 expression in healthy horses and horses with severe asthma.American journal of veterinary research · 2022Article
- Expression of immune response genes in human corneal epithelial cells interacting with Aspergillus flavus conidia.BMC genomics · 2022Article
- Epigenetic regulation of epithelial dectin-1 through an IL-33-STAT3 axis in allergic disease.Allergy · 2022Article
- Aspergillus fumigatus Induces the Release of IL-8 and MCP-1 by Activating Nuclear Transcription Through Dectin-1 and CR3 Receptors in Alveolar Epithelial Cells.Current microbiology · 2021Article
- The Role of SREC-Ⅰ in Innate Immunity to Aspergillus fumigatus Keratitis.Investigative ophthalmology & visual science · 2021Article
- Human Corneal Epithelial Cells Internalize Aspergillus flavus Spores by Actin-Mediated Endocytosis.Infection and immunity · 2021Article
- Isorhamnetin Ameliorates Aspergillus fumigatus Keratitis by Reducing Fungal Load, Inhibiting Pattern-Recognition Receptors and Inflammatory Cytokines.Investigative ophthalmology & visual science · 2021Article
- The sino-nasal warzone: transcriptomic and genomic studies on sino-nasal aspergillosis in dogs.NPJ biofilms and microbiomes · 2020Article
- Honokiol Reduces Fungal Load, Toll-Like Receptor-2, and Inflammatory Cytokines in Aspergillus fumigatus Keratitis.Investigative ophthalmology & visual science · 2020Article
- Article
- Expression of macrophage migration inhibitory factor inInternational journal of ophthalmology · 2019Article
- Phospholipase Cγ2 is critical for CaBMC ophthalmology · 2018Article
- Production of interleukin-1β related to mammalian target of rapamycin/Toll-like receptor 4 signaling pathway duringInternational journal of ophthalmology · 2018Article
- Article
- Role of Pattern Recognition Receptors in the Modulation of Antimicrobial Peptide Expression in the Corneal Epithelial Innate Response to F. solani.Investigative ophthalmology & visual science · 2017Article
- Interactions of Aspergillus fumigatus Conidia with Airway Epithelial Cells: A Critical Review.Frontiers in microbiology · 2016Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo identify whether Aspergillus fumigatus (A. fumigatus) hyphae antigens induced the release of interleukin-8 (IL-8) in anti-fungal innate immunity of cultured human corneal epithelial cells (HCECs) and determine the involvement of intracellular signalling pathways.
methodsHCECs were treated with A. fumigatus hyphae antigens with different concentrations and time. The cytoplasmic calcium of HCECs were assessed by fluorescence imaging. Western blot was used to detect the expression of Ca(2+)-dependent protein kinase C (PKC). The IL-8 levels were determined by specific human IL-8 enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase polymerase chain reaction (RT-PCR). Using a series of pharmacological inhibitors, we examined the upstream signalling pathway responsible for IL-8 expression in response to A. fumigatus hyphae antigens.
resultsCells exposed to A. fumigatus hyphae antigens showed higher level of IL-8 mRNA expression and protein production. We demonstrated here that stimulation of HCECs with A. fumigatus hyphae triggers an intracellular Ca(2+) flux and results in the activation of Ca(2+)-dependent PKC (α, βI and βII) which can be attenuated by pre-treatment of cells with laminarin, suggesting that Dectin-1 signals pathway induced cytoplasmic calcium and influence the activation of PKC in HCECs. Inhibitors of Ca(2+)-dependent PKC (Ro-31-8220 and Go6976) significantly abolished hyphae-induced expression of IL-8.
conclusionOur findings suggest that A. fumigatus hyphae-induced IL-8 expression was regulated by the activation of Dectin-1-mediated Ca(2+)-dependent PKC in HCECs.
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