The Use of Atomic Force Microscopy for 3D Analysis of Nucleic Acid Hybridization on Microarrays.

E V Dubrovin, G V Presnova, M Yu Rubtsova, A M Egorov, V G Grigorenko, I V Yaminsky

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Article in Acta naturae. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

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3citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

Multiplex Digital Quantification of β-Lactamase Genes in Antibiotic-Resistant Bacteria by Counting Gold Nanoparticle Labels on Silicon Microchips.Biosensors · 2022
Article
Organic and inorganic mixed phase modification of a silver surface for functionalization with biomolecules and stabilization of electromotive force.RSC advances · 2021
Article
From the Clinical Problem to the Basic Research-Co-Culture Models of Osteoblasts and Osteoclasts.International journal of molecular sciences · 2018
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5 · Who and what money

Authors and funding

6 authors.

E V DubrovinDepartment of Physics, Lomonosov Moscow State University, Leninskie gory, 1/2, 119991, Moscow, Russia.

G V PresnovaDepartment of Chemistry, Lomonosov Moscow State University, Leninskie gory, 1/3, 119991, Moscow, Russia.

M Yu RubtsovaDepartment of Chemistry, Lomonosov Moscow State University, Leninskie gory, 1/3, 119991, Moscow, Russia.

A M EgorovDepartment of Chemistry, Lomonosov Moscow State University, Leninskie gory, 1/3, 119991, Moscow, Russia ; Russian Medical Academy of Postgraduate Education, Barrikadnaya, 2/1, 125993, Moscow, Russia.

V G GrigorenkoDepartment of Chemistry, Lomonosov Moscow State University, Leninskie gory, 1/3, 119991, Moscow, Russia.

I V YaminskyDepartment of Physics, Lomonosov Moscow State University, Leninskie gory, 1/2, 119991, Moscow, Russia ; Department of Chemistry, Lomonosov Moscow State University, Leninskie gory, 1/3, 119991, Moscow, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oligonucleotide microarrays are considered today to be one of the most efficient methods of gene diagnostics. The capability of atomic force microscopy (AFM) to characterize the three-dimensional morphology of single molecules on a surface allows one to use it as an effective tool for the 3D analysis of a microarray for the detection of nucleic acids. The high resolution of AFM offers ways to decrease the detection threshold of target DNA and increase the signal-to-noise ratio. In this work, we suggest an approach to the evaluation of the results of hybridization of gold nanoparticle-labeled nucleic acids on silicon microarrays based on an AFM analysis of the surface both in air and in liquid which takes into account of their three-dimensional structure. We suggest a quantitative measure of the hybridization results which is based on the fraction of the surface area occupied by the nanoparticles.

Indexed as

atomic force microscopyCTXM type β-lactamasesDNAgold nanoparticleshybridizationoligonucleotide microarrays

Identifiers

PMID26085952

PMCPMC4463420

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.