ArticleBasic and clinical andrology2015
Impact on ICSI outcomes of adding 24 h of in vitro culture before testicular sperm freezing: a retrospective study.
Article in Basic and clinical andrology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Could cleaved embryo morphology and morphokinetics be associated with prenatal and neonatal outcomes?Journal of assisted reproduction and genetics · 2025Article
- A new insight into male fertility preservation for patients with completely immotile spermatozoa.Reproductive biology and endocrinology : RB&E · 2017Article
- Improvement of motility after culture of testicular spermatozoa: the effects of incubation timing and temperature.Translational andrology and urology · 2017Article
- A successful pregnancy using completely immotile but viable frozen-thawed spermatozoa selected by laser.Clinical and experimental reproductive medicine · 2017Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo compare sperm parameters and intracytoplasmic sperm injection (ICSI) outcomes for testicular spermatozoa frozen on the day of the biopsy (DO) with those frozen after 24 h of in vitro culture (D1).
methodsIn this retrospective study, from 1999 to 2012, forty-nine azoospermic patients were included to compare sperm (motility and viability) and outcomes (fertilization (FR), implantation (IR), pregnancy (PR) and delivery rates (DR)).
resultsThe in vitro culture increased total motility (+2.8 %, p = 0.0161) but decreased viability (-8.3 %, p = 0.007). After 24 h of culture, the post-thaw changes in motility and viability were not significant. Twenty-six couples underwent ICSI: thirty-four ICSI were performed with spermatozoa cryopreserved at D0 and eighteen with spermatozoa frozen at D1. Cumulated IR and DR were lower for ICSI with D1 spermatozoa than with D0 spermatozoa (IR: 21.6 % with D0 vs. 9.8 % with D1, p = 0.102; DR: 27.5 % with D0 vs. 8.3 % with D1, p = 0.049).
conclusionDespite improving motility, freezing spermatozoa 24 h after testicular biopsy had a potential negative effect on ICSI outcomes, notably on delivery rates. These results may be related to the detrimental impact of the additional culture on the nuclear integrity of sperm.
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