ArticleJournal of clinical microbiology2015
Field-Applicable Recombinase Polymerase Amplification Assay for Rapid Detection of Mycoplasma capricolum subsp. capripneumoniae.
Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.
- Co-occurrence of contagious caprine pleuropneumonia and pasteurellosis in goats of sub-Saharan region: systematic review.Tropical animal health and production · 2024Pooled it
- Development of RT-RPA assays for rapid detection of mango-infecting viruses using high-throughput sequencing-derived genomic information.BMC plant biology · 2026Article
- Establishment of methods for visual and rapid detection of piscine lactococcosis based on isothermal recombinase polymerase amplification.BMC veterinary research · 2026Article
- Recombinase polymerase amplification of forensic short tandem repeat loci.Scientific reports · 2025Article
- Laboratory tools for the direct detection of bacterial respiratory infections and antimicrobial resistance: a scoping review.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Rapid lateral flow immunoassay for fluorescence detection of canine distemper virus (CDV).Frontiers in veterinary science · 2024Article
- Establishment of a recombinase polymerase amplification (RPA) fluorescence assay for the detection of swine acute diarrhea syndrome coronavirus (SADS-CoV).BMC veterinary research · 2022Article
- Establishment and Application of a Real-Time Recombinase Polymerase Amplification Assay for the Detection of Avian Leukosis Virus Subgroup J.Frontiers in veterinary science · 2022Article
- Molecular Detection of Feline Coronavirus Based on Recombinase Polymerase Amplification Assay.Pathogens (Basel, Switzerland) · 2021Article
- On point identification of species origin of food animals by recombinase polymerase amplification-lateral flow (RPA-LF) assay targeting mitochondrial gene sequences.Journal of food science and technology · 2021Article
- Development of Immunochromatographic Assay for the Rapid Detection ofFrontiers in microbiology · 2021Article
- Recombinase polymerase amplification assay combined with a dipstick-readout for rapid detection of Mycoplasma ovipneumoniae infections.PloS one · 2021Article
- Development of a recombinase polymerase amplification fluorescence assay to detect feline coronavirus.Molecular and cellular probes · 2020Article
- Development of a reverse transcription recombinase polymerase based isothermal amplification coupled with lateral flow immunochromatographic assay (CTV-RT-RPA-LFICA) for rapid detection of Citrus tristeza virus.Scientific reports · 2020Article
- A whole-genome worldwide molecular epidemiology approach for contagious caprine pleuropneumonia.Heliyon · 2020Article
- Contagious caprine pleuropneumonia - a comprehensive review.The veterinary quarterly · 2019Review
- Establishment of a recombinase polymerase amplification (RPA) assay for the detection of Brucella spp. Infection.Molecular and cellular probes · 2019Article
- Use of a recombinase polymerase amplification commercial kit for rapid visual detection of Pasteurella multocida.BMC veterinary research · 2019Article
- In vivo role of capsular polysaccharide in Mycoplasma mycoides.The Journal of infectious diseases · 2019Article
- Reproduction of contagious caprine pleuropneumonia reveals the ability of convalescent sera to reduce hydrogen peroxide production in vitro.Veterinary research · 2019Article
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Contagious caprine pleuropneumonia (CCPP) is a highly contagious disease caused by Mycoplasma capricolum subsp. capripneumoniae that affects goats in Africa and Asia. Current available methods for the diagnosis of Mycoplasma infection, including cultivation, serological assays, and PCR, are time-consuming and require fully equipped stationary laboratories, which make them incompatible with testing in the resource-poor settings that are most relevant to this disease. We report a rapid, specific, and sensitive assay employing isothermal DNA amplification using recombinase polymerase amplification (RPA) for the detection of M. capricolum subsp. capripneumoniae. We developed the assay using a specific target sequence in M. capricolum subsp. capripneumoniae, as found in the genome sequence of the field strain ILRI181 and the type strain F38 and that was further evidenced in 10 field strains from different geographical regions. Detection limits corresponding to 5 × 10(3) and 5 × 10(4) cells/ml were obtained using genomic DNA and bacterial culture from M. capricolum subsp. capripneumoniae strain ILRI181, while no amplification was obtained from 71 related Mycoplasma isolates or from the Acholeplasma or the Pasteurella isolates, demonstrating a high degree of specificity. The assay produces a fluorescent signal within 15 to 20 min and worked well using pleural fluid obtained directly from CCPP-positive animals without prior DNA extraction. We demonstrate that the diagnosis of CCPP can be achieved, with a short sample preparation time and a simple read-out device that can be powered by a car battery, in <45 min in a simulated field setting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.