ArticleMolecular biology of the cell2015
Reduced tubulin polyglutamylation suppresses flagellar shortness in Chlamydomonas.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 57 citations in OpenAlex.
- Tubulin tyrosination/detyrosination regulate the affinity and sorting of intraflagellar transport trains on axonemal microtubule doublets.Nature communications · 2025Article
- Distribution and bulk flow analyses of the intraflagellar transport (IFT) motor kinesin-2 support an "on-demand" model for Chlamydomonas ciliary length control.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Protofilament-specific nanopatterns of tubulin post-translational modifications regulate the mechanics of ciliary beating.Current biology : CB · 2024Article
- Article
- Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii.PLoS genetics · 2024Article
- Outer-arm dynein light chain LC1 is required for normal motor assembly kinetics, ciliary stability, and motility.Molecular biology of the cell · 2023Article
- Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly.Cell research · 2022Article
- The Emerging Roles of Axonemal Glutamylation in Regulation of Cilia Architecture and Functions.Frontiers in cell and developmental biology · 2021Review
- Mutation of NEKL-4/NEK10 and TTLL genes suppress neuronal ciliary degeneration caused by loss of CCPP-1 deglutamylase function.PLoS genetics · 2020Article
- Speed and Diffusion of Kinesin-2 Are Competing Limiting Factors in Flagellar Length-Control Model.Biophysical journal · 2020Article
- Asymmetries in the cilia ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Cilia Loss and Dynein Assembly Defects in Planaria Lacking an Outer Dynein Arm-Docking Complex Subunit.Zoological science · 2020Article
- WDR92 is required for axonemal dynein heavy chain stability in cytoplasm.Molecular biology of the cell · 2019Article
- LF4/MOK and a CDK-related kinase regulate the number and length of cilia in Tetrahymena.PLoS genetics · 2019Article
- The dynamic and structural properties of axonemal tubulins support the high length stability of cilia.Nature communications · 2019Article
- O-GlcNAcylation Regulates Primary Ciliary Length by Promoting Microtubule Disassembly.iScience · 2019Article
- RPGRIP1L helps to establish the ciliary gate for entry of proteins.Journal of cell science · 2018Article
- Cilium structure, assembly, and disassembly regulated by the cytoskeleton.The Biochemical journal · 2018Review
- A microtubule-dynein tethering complex regulates the axonemal inner dynein f (I1).Molecular biology of the cell · 2018Article
- General and specific promotion of flagellar assembly by a flagellar nucleoside diphosphate kinase.Molecular biology of the cell · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Ciliary length control is an incompletely understood process essential for normal ciliary function. The flagella of Chlamydomonas mutants lacking multiple axonemal dyneins are shorter than normal; previously it was shown that this shortness can be suppressed by the mutation suppressor of shortness 1 (ssh1) via an unknown mechanism. To elucidate this mechanism, we carried out genetic analysis of ssh1 and found that it is a new allele of TPG2 (hereafter tpg2-3), which encodes FAP234 functioning in tubulin polyglutamylation in the axoneme. Similar to the polyglutamylation-deficient mutants tpg1 and tpg2-1, tpg2-3 axonemal tubulin has a greatly reduced level of long polyglutamate side chains. We found that tpg1 and tpg2-1 mutations also promote flagellar elongation in short-flagella mutants, consistent with a polyglutamylation-dependent mechanism of suppression. Double mutants of tpg1 or tpg2-1 and fla10-1, a temperature-sensitive mutant of intraflagellar transport, underwent slower flagellar shortening than fla10-1 at restrictive temperatures, indicating that the rate of tubulin disassembly is decreased in the polyglutamylation-deficient flagella. Moreover, α-tubulin incorporation into the flagellar tips in temporary dikaryons was retarded in polyglutamylation-deficient flagella. These results show that polyglutamylation deficiency stabilizes axonemal microtubules, decelerating axonemal disassembly at the flagellar tip and shifting the axonemal assembly/disassembly balance toward assembly.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.