ArticleJournal of neurophysiology2015
MATLAB-based automated patch-clamp system for awake behaving mice.
Article in Journal of neurophysiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- A robotic patch-clamp system with real-time localization and phase-synchronized capture of dynamic in vivo cells using micropipette resistance modelling.Microsystems & nanoengineering · 2026Article
- Vasopressin and oxytocin excite BNST neurons via oxytocin receptors, which reduce anxious arousal.Cell reports · 2025Article
- Neuron Contact Detection Based on Pipette Precise Positioning for Robotic Brain-Slice Patch Clamps.Sensors (Basel, Switzerland) · 2023Article
- Distorted neurocomputation by a small number of extra-large spines in psychiatric disorders.Science advances · 2023Article
- Automated microscope-independent fluorescence-guided micropipette.Biomedical optics express · 2021Article
- Automatic deep learning-driven label-free image-guided patch clamp system.Nature communications · 2021Article
- Single neuron recording: progress towards high-throughput analysis.Bioelectronics in medicine · 2020Article
- Engineering microscale systems for fully autonomous intracellular neural interfaces.Microsystems & nanoengineering · 2020Article
- Article
- Robotic platform for microinjection into single cells in brain tissue.EMBO reports · 2019Article
- Autonomous patch-clamp robot for functional characterization of neurons in vivo: development and application to mouse visual cortex.Journal of neurophysiology · 2019Article
- Craniobot: A computer numerical controlled robot for cranial microsurgeries.Scientific reports · 2019Article
- Robotic navigation to subcortical neural tissue for intracellular electrophysiology in vivo.Journal of neurophysiology · 2017Article
- Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices.Journal of visualized experiments : JoVE · 2017Article
- A robot for high yield electrophysiology and morphology of single neurons in vivo.Nature communications · 2017Article
- Cleaning patch-clamp pipettes for immediate reuse.Scientific reports · 2016Article
- Integration of autopatching with automated pipette and cell detection in vitro.Journal of neurophysiology · 2016Article
- Robots record brain activity inside neurons.Nature · 2016Article
- 3D Image-Guided Automatic Pipette Positioning for Single Cell Experiments in vivo.Scientific reports · 2015Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Automation has been an important part of biomedical research for decades, and the use of automated and robotic systems is now standard for such tasks as DNA sequencing, microfluidics, and high-throughput screening. Recently, Kodandaramaiah and colleagues (Nat Methods 9: 585-587, 2012) demonstrated, using anesthetized animals, the feasibility of automating blind patch-clamp recordings in vivo. Blind patch is a good target for automation because it is a complex yet highly stereotyped process that revolves around analysis of a single signal (electrode impedance) and movement along a single axis. Here, we introduce an automated system for blind patch-clamp recordings from awake, head-fixed mice running on a wheel. In its design, we were guided by 3 requirements: easy-to-use and easy-to-modify software; seamless integration of behavioral equipment; and efficient use of time. The resulting system employs equipment that is standard for patch recording rigs, moderately priced, or simple to make. It is written entirely in MATLAB, a programming environment that has an enormous user base in the neuroscience community and many available resources for analysis and instrument control. Using this system, we obtained 19 whole cell patch recordings from neurons in the prefrontal cortex of awake mice, aged 8-9 wk. Successful recordings had series resistances that averaged 52 ± 4 MΩ and required 5.7 ± 0.6 attempts to obtain. These numbers are comparable with those of experienced electrophysiologists working manually, and this system, written in a simple and familiar language, will be useful to many cellular electrophysiologists who wish to study awake behaving mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.