ArticleOtolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery2015
Dysregulated Macrophages Are Present in Bleomycin-Induced Murine Laryngotracheal Stenosis.
Article in Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 52 citations in OpenAlex.
- Is COVID-19 Infection A Risk Factor for Intubation-Related Acquired Airway Stenosis?Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2026Article
- A Novel Murine Model of Acute Laryngeal Injury After Intubation.The Laryngoscope · 2026Article
- Synergistic Effects of Erythromycin and Budesonide on the Mitigation of Tracheal Stenosis Associated With Macrophage Phenotype Alteration and HDAC2 Upregulation.Canadian respiratory journal · 2026Article
- Novel murine partial tracheal occlusion model with altered respiratory dynamics.American journal of physiology. Lung cellular and molecular physiology · 2025Article
- Preclinical Vocal Fold and Airway Injury Models: A Scoping Review.Laryngoscope investigative otolaryngology · 2025Review
- Dynamic single-cell transcriptomic reveals the cellular heterogeneity and a novel fibroblast subpopulation in laryngotracheal stenosis.Biology direct · 2025Article
- Unveiling Vaginal Fibrosis: A Novel Murine Model Using Bleomycin and Epithelial Disruption.Open journal of obstetrics and gynecology · 2025Article
- Review
- Macrophage STING signaling promotes fibrosis in benign airway stenosis via an IL6-STAT3 pathway.Nature communications · 2025Article
- An Ovine Model Yields Histology and Gene Expression Changes Consistent with Laryngotracheal Stenosis.The Laryngoscope · 2024Article
- Localized delivery of therapeutics impact laryngeal mechanics, local inflammatory response, and respiratory microbiome following upper airway intubation injury in swine.Respiratory research · 2024Article
- Murine Model of Airway Fibrosis has Anatomic, Physiologic, and Molecular Congruency to Human iSGS.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2024Article
- Localizing Hormone Receptor Expression to Cellular Compartments in Idiopathic Subglottic Stenosis.The Laryngoscope · 2023Article
- Article
- Amelioration of Subglottic Stenosis by Antimicrobial Peptide Eluting Endotracheal Tubes.Cellular and molecular bioengineering · 2023Article
- The role of tracheal wall injury in the development of benign airway stenosis in rabbits.Scientific reports · 2023Article
- Laryngotracheal stenosis: Mechanistic review.Head & neck · 2022Review
- Tracheal Macrophages During Regeneration and Repair of Long-Segment Airway Defects.The Laryngoscope · 2022Article
- Molecular Mechanisms and Physiological Changes behind Benign Tracheal and Subglottic Stenosis in Adults.International journal of molecular sciences · 2022Review
- Glutamine Inhibition Reduces Iatrogenic Laryngotracheal Stenosis.The Laryngoscope · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveTo define the inflammatory cell infiltrate preceding fibrosis in a laryngotracheal stenosis (LTS) murine model. STUDY
designProspective controlled murine study.
settingLaboratory. SUBJECTS AND
methodsChemomechanical injury mice (n = 44) sustained bleomycin-coated wire-brush injury to the laryngotracheal complex while mechanical injury controls (n = 42) underwent phosphate-buffered saline (PBS)-coated wire-brush injury. Mock surgery controls (n = 34) underwent anterior transcervical tracheal exposure only. Inflammatory and fibrosis protein and gene expression were assessed in each condition. Immunohistochemistry served as a secondary outcome.
resultsIn chemomechanical injury mice, there was an upregulation of collagen I (P < .0001, P < .0001), Tgf-β (P = .0023, P = .0008), and elastin (P < .0001, P < .0001) on day 7; acute inflammatory gene Il1β (P = .0027, P = .0008) on day 1; and macrophage gene CD11b (P = .0026, P = .0033) on day 1 vs mechanical and mock controls, respectively. M1 marker inducible nitric oxide synthase (iNOS) expression decreased (P = .0014) while M2 marker Arg1 (P = .0002) increased on day 7 compared with mechanical controls. Flow cytometry demonstrated increased macrophages (P = .0058, day 4) and M1 macrophages (P = .0148, day 4; P = .0343, day 7; P = .0229, day 10) compared to mock controls. There were similarities between chemomechanical and mechanical injury mice with an increase in M2 macrophages at day 10 (P = .0196).
conclusionsThe bleomycin-induced LTS mouse model demonstrated increased macrophages involved with the development of fibrosis. Macrophage immunophenotype suggested that dysregulated M2 macrophages have a role in abnormal laryngotracheal wound healing. These data delineate inflammatory cells and signaling pathways in LTS that may potentially be modulated to lessen fibroblast proliferation and collagen deposition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.