Evidence map›Paper›PMID 26083920›Full record

ArticleBiophysical journal2015

Deconvolution-free Subcellular Imaging with Axially Swept Light Sheet Microscopy.

Kevin M Dean, Philippe Roudot, Erik S Welf, Gaudenz Danuser, Reto Fiolka

Abstract read
In one paragraph

Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 136 papers.

0numbers the graph read from it
0cells of the map it votes in
136citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

136 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
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  5. Article
  6. Axially Swept Light-Sheet Microscopy using scattering and fluorescence contrast mechanisms.Proceedings of SPIE--the International Society for Optical Engineering · 2026
    Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Refractive Index-Corrected Light-Sheet Microscopy for Macro-View Cardiovascular Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
  13. Article
  14. Cutting-edge technologies in neural regeneration.Cell regeneration (London, England) · 2025
    Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Review

76 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kevin M DeanDepartment of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas.
Philippe RoudotDepartment of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas.
Erik S WelfDepartment of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas.
Gaudenz DanuserDepartment of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas.
Reto FiolkaDepartment of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas. Electronic address: reto.fiolka@utsouthwestern.edu.

Funding

Structural basis of the specific protein-protein interactions underlying IF assemP01GM096971 · NIGMS · NORTHWESTERN UNIVERSITY AT CHICAGO · PI GOLDMAN, ROBERT D · 2011 to 2021
$16.8M
NIGMS NIH HHS P01 GM096971
6 · The paper itself

Abstract

The use of propagation invariant Bessel beams has enabled high-resolution subcellular light sheet fluorescence microscopy. However, the energy within the concentric side lobe structure of Bessel beams increases significantly with propagation length, generating unwanted out-of-focus fluorescence that enforces practical limits on the imaging field of view size. Here, we present a light sheet fluorescence microscope that achieves 390 nm isotropic resolution and high optical sectioning strength (i.e., out-of-focus blur is strongly suppressed) over large field of views, without the need for structured illumination or deconvolution-based postprocessing. We demonstrate simultaneous dual-color, high-contrast, and high-dynamic-range time-lapse imaging of migrating cells in complex three-dimensional microenvironments, three-dimensional tracking of clathrin-coated pits, and long-term imaging spanning >10 h and encompassing >2600 time points.

Indexed as

Cell Culture TechniquesCell MovementGreen Fluorescent ProteinsHumansImaging, Three-DimensionalMicroscopy, FluorescenceOptical ImagingRetinal Pigment EpitheliumTime-Lapse ImagingGreen Fluorescent Proteins

Identifiers

PMID26083920
PMCPMC4472079

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.