ArticlePloS one2015
Changes in the Gene Expression Profiles of the Hypopharyngeal Gland of Worker Honeybees in Association with Worker Behavior and Hormonal Factors.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- Flavonoid constituents contribute to the low diastase activity ofFood chemistry: X · 2026Article
- Evolution of the α-Glucosidase Gene Family in Apoidea.Ecology and evolution · 2026Article
- Impact of Monofloral Pollen Diets on the Development of Hypopharyngeal Glands and Modulation of Enzymatic, Non-Enzymatic, and Ionic Biomarker Activities in Selected Fat Body Segments and Hemolymph ofMolecules (Basel, Switzerland) · 2026Article
- Organic Acid Supplementation in Worker Honeybees (Insects · 2025Review
- Neurotranscriptomic profiling of deformed wing virus-infected honey bee foragers (Apis mellifera) with different cognitive abilities.Biology open · 2025Article
- Selection and validation of RT-qPCR reference genes for multi-tissue gene expression normalization in two honeybee subspecies across post-emergence developmental stages.BMC genomics · 2025Article
- Cognition from genes to ecology: individual differences incognition and its potential role in a social network.Animal cognition · 2025Review
- In the battle of survival: transcriptome analysis of hypopharyngeal gland of the Apis mellifera under temperature-stress.BMC genomics · 2025Article
- Stress drives premature hive exiting behavior that leads to death in young honey bee (Apis mellifera) workers.Biological research · 2024Article
- Novel fungicide and neonicotinoid insecticide impair flight behavior in pollen foraging honey bees, Apis mellifera.Scientific reports · 2024Article
- Metabolism gene expression in worker honey bees after exposure to 50Hz electric field - semi-field analysis.Frontiers in zoology · 2024Article
- Gut microbiota contribute to variations in honey bee foraging intensity.The ISME journal · 2024Article
- Expression of subunits of an insecticide target receptor varies across tissues, life stages, castes, and species of social bees.Molecular ecology · 2023Article
- Expression Profile of microRNAs during Development of the Hypopharyngeal Gland in Honey Bee,International journal of molecular sciences · 2022Article
- Age and Behavior-Dependent Differential miRNAs Expression in the Hypopharyngeal Glands of Honeybees (Insects · 2021Article
- Correlation Between Increased Homing Flight Duration and Altered Gene Expression in the Brain of Honey Bee Foragers After Acute Oral Exposure to Thiacloprid and Thiamethoxam.Frontiers in insect science · 2021Article
- Transcriptional Control of Honey Bee (Insects · 2018Article
- Morphogenesis of honeybee hypopharyngeal gland during pupal development.Frontiers in zoology · 2017Article
- Preparation of Single-cohort Colonies and Hormone Treatment of Worker Honeybees to Analyze Physiology Associated with Role and/or Endocrine System.Journal of visualized experiments : JoVE · 2016Article
- α-Glucosidases and α-1,4-glucan lyases: structures, functions, and physiological actions.Cellular and molecular life sciences : CMLS · 2016Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hypopharyngeal glands (HPGs) of worker honeybees undergo physiological changes along with the age-dependent role change from nursing to foraging: nurse bee HPGs secrete mainly major royal jelly proteins, whereas forager HPGs secrete mainly α-glucosidase III, which converts the sucrose in the nectar into glucose and fructose. We previously identified two other genes, Apis mellifera buffy (Ambuffy) and Apis mellifera matrix metalloproteinase 1 (AmMMP1), with enriched expression in nurse bee and forager HPGs, respectively. In the present study, to clarify the molecular mechanisms that coordinate HPG physiology with worker behavior, we first analyzed whether Ambuffy, AmMMP1, mrjp2 (a gene encoding one of major royal jelly protein isoforms), and Hbg3 (a gene encoding α-glucosidase III) expression, is associated with worker behavior in 'single-cohort colonies' where workers of almost the same age perform different tasks. Expression of these genes correlated with the worker's role, while controlling for age, indicating their regulation associated with the worker's behavior. Associated gene expression suggested the possible involvement of some hormonal factors in its regulation. We therefore examined the relationship between ecdysone- and juvenile hormone (JH)-signaling, and the expression profiles of these 'indicator' genes (nurse bee HPG-selective genes: mrjp2 and Ambuffy, and forager HPG-selective genes: Hbg3 and AmMMP1). Expression of both ecdysone-regulated genes (ecdysone receptor, mushroom body large type Kenyon cell specific protein-1, and E74) and JH-regulated genes (Methoprene tolerant and Krüppel homolog 1) was higher in the forager HPGs than in the nurse bee HPGs, suggesting the possible roles of ecdysone- and JH-regulated genes in worker HPGs. Furthermore, 20-hydroxyecdysone-treatment repressed both nurse bee- and forager-selective gene expression, whereas methoprene-treatment enhanced the expression of forager-selective genes and repressed nurse bee-selective genes in the HPGs. Our findings suggest that both ecdysone- and JH-signaling cooperatively regulate the physiological state of the HPGs in association with the worker's behavior.
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