ArticlePloS one2015
Isolation of High-Quality Total RNA from Chinese Fir (Cunninghamia lanceolata (Lamb.) Hook).
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 30 citations in OpenAlex.
- Genetic diversity and distribution pattern responses ofFrontiers in plant science · 2026Article
- A complex interplay of genetic introgression and local adaptation during the evolutionary history of three closely related spruce species.Plant diversity · 2025Article
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- Isolation of high-quality RNA for high throughput applications from secondary metabolite-rich Crocus sativus L.BMC research notes · 2022Article
- An optimized method to obtain high-quality RNA from different tissues in Lilium davidii var. unicolor.Scientific reports · 2022Article
- Article
- Protocols for the Analysis of microRNA Expression, Biogenesis, and Function in Immune Cells.Current protocols in immunology · 2019Article
- A phenol/chloroform-free method to extract nucleic acids from recalcitrant, woody tropical species for gene expression and sequencing.Plant methods · 2019Article
- Comparative analysis and innovation of a simple and rapid method for high-quality RNA and DNA extraction of kiwifruit.MethodsX · 2018Article
- A method for extracting high-quality total RNA from plant rich in polysaccharides and polyphenols using Dendrobium huoshanense.PloS one · 2018Article
- Comparative assessment of four RNA extraction methods and modification to obtain high-quality RNA from3 Biotech · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA isolation with RNA in a high quantity is a basic analytical method in plant genetics, molecular biology and related physiological investigations. To understand the genetic and molecular biology of Chinese fir, sufficient high-quality total RNA must be obtained for cDNA library construction and other downstream molecular applications. However, extracting RNA from Chinese fir is difficult and often requires the modification of existing protocols. Chinese fir tissues containing large amounts of polysaccharides and polyphenol compounds and are one of the most difficult plant tissues for RNA isolation. Therefore, we developed a simple method for extracting high-quality RNA from Chinese fir tissues. RNA isolations were performed within two hours, RNA quality was measured for yield and purity. Total RNA obtained from this procedure was successfully used for cDNA library construction, RT-PCR and transcriptome sequencing. It was proven that extracted RNA was intact and suitable for downstream molecular applications, including RT-PCR and qPCR, and other downstream molecular applications. Thus, this protocol represents a simple, efficient, and low-cost method.
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