ArticleeNeuro
Measurement of Retinal Blood Flow Using Fluorescently Labeled Red Blood Cells.
Article in eNeuro. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- Understanding the Impact of Synthetic Hematocrit Levels and Biomimetic Channel Widths on Bubble Parameters in Vascular Systems on a Chip.Biomimetics (Basel, Switzerland) · 2025Article
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- The lipoprotein lipase that is shuttled into capillaries by GPIHBP1 enters the glycocalyx where it mediates lipoprotein processing.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Variability in Capillary Perfusion Is Increased in Regions of Retinal Ischemia Due to Branch Retinal Vein Occlusion.Investigative ophthalmology & visual science · 2023Article
- Differential reductions in the capillary red-blood-cell flux between retina and brain under chronic global hypoperfusion.Neurophotonics · 2023Article
- Super-Resolution Ultrasound Localization Microscopy Using High-Frequency Ultrasound to Measure Ocular Perfusion Velocity in the Rat Eye.Bioengineering (Basel, Switzerland) · 2023Article
- Fast volumetric imaging with line-scan confocal microscopy by electrically tunable lens at resonant frequency.Optics express · 2022Article
- In Vivo Capillary Structure and Blood Cell Flux in the Normal and Diabetic Mouse Eye.Investigative ophthalmology & visual science · 2022Article
- Measurement of Blood Velocity With Laser Scanning Microscopy: Modeling and Comparison of Line-Scan Image-Processing Algorithms.Frontiers in physiology · 2022Article
- Methods to measure blood flow and vascular reactivity in the retina.Frontiers in medicine · 2022Review
- A Permanent Window for Investigating Cancer Metastasis to the Lung.Journal of visualized experiments : JoVE · 2021Article
- Labeling of Erythrocytes by Porphyrin-Phospholipid.Advanced nanobiomed research · 2021Article
- Imaging volumetric dynamics at high speed in mouse and zebrafish brain with confocal light field microscopy.Nature biotechnology · 2021Article
- Article
- Caffeine Delays Retinal Neurovascular Coupling during Dark to Light Adaptation in Healthy Eyes Revealed by Optical Coherence Tomography Angiography.Investigative ophthalmology & visual science · 2020Article
- Article
- Unbiased Analysis Method for Measurement of Red Blood Cell Size and Velocity With Laser Scanning Microscopy.Frontiers in neuroscience · 2019Article
- Rodent retinal circulation organization and oxygen metabolism revealed by visible-light optical coherence tomography.Biomedical optics express · 2018Article
- NoninvasiveBiomedical optics express · 2018Article
- Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo.Journal of visualized experiments : JoVE · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Blood flow is a useful indicator of the metabolic state of the retina. However, accurate measurement of retinal blood flow is difficult to achieve in practice. Most existing optical techniques used for measuring blood flow require complex assumptions and calculations. We describe here a simple and direct method for calculating absolute blood flow in vessels of all sizes in the rat retina. The method relies on ultrafast confocal line scans to track the passage of fluorescently labeled red blood cells (fRBCs). The accuracy of the blood flow measurements was verified by (1) comparing blood flow calculated independently using either flux or velocity combined with diameter measurements, (2) measuring total retinal blood flow in arterioles and venules, (3) measuring blood flow at vessel branch points, and (4) measuring changes in blood flow in response to hyperoxic and hypercapnic challenge. Confocal line scans oriented parallel and diagonal to vessels were used to compute fRBC velocity and to examine velocity profiles across the width of vessels. We demonstrate that these methods provide accurate measures of absolute blood flow and velocity in retinal vessels of all sizes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.