ArticleApplications in plant sciences2015
Plastid primers for angiosperm phylogenetics and phylogeography.
Article in Applications in plant sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
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- Molecular Characterization of Wild and Cultivated Strawberry (Genetics research · 2022Article
- The Conservation of Chloroplast Genome Structure and Improved Resolution of Infrafamilial Relationships of Crassulaceae.Frontiers in plant science · 2021Article
- A comparative plastomics approach reveals available molecular markers for the phylogeographic study ofEcology and evolution · 2020Article
- Two new species and a new species record ofPhytoKeys · 2020Article
- Plastome Evolution inFrontiers in plant science · 2020Article
- Plastome phylogenomics of Saussurea (Asteraceae: Cardueae).BMC plant biology · 2019Article
- Integral Phylogenomic Approach over Ilex L. Species from Southern South America.Life (Basel, Switzerland) · 2017Article
- The complete chloroplast genome of Primulina and two novel strategies for development of high polymorphic loci for population genetic and phylogenetic studies.BMC evolutionary biology · 2017Article
- Strategies for complete plastid genome sequencing.Molecular ecology resources · 2017Review
- Lineage-specific evolutionary rate in plants: Contributions of a screening for Cereus (Cactaceae).Applications in plant sciences · 2016Article
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1 author.
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Abstract
premise of the studyPCR primers are available for virtually every region of the plastid genome. Selection of which primer pairs to use is second only to selection of the genic region. This is particularly true for research at the species/population interface.
methodsPrimer pairs for 130 regions of the chloroplast genome were evaluated in 12 species distributed across the angiosperms. Likelihood of amplification success was inferred based upon number and location of mismatches to target sequence. Intraspecific sequence variability was evaluated under three different criteria in four species.
resultsMany published primer pairs should work across all taxa sampled, with the exception of failure due to genomic reorganization events. Universal barcoding primers were the least likely to work (65% success). The list of most variable regions for use within species has little in common with the lists identified in prior studies. DISCUSSION: Published primer sequences should amplify a diversity of flowering plant DNAs, even those designed for specific taxonomic groups. "Universal" primers may have extremely limited utility. There was little consistency in likelihood of amplification success for any given publication across lineages or within lineage across publications.
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