ArticleNucleic acids research2015
Decoupling of divergent gene regulation by sequence-specific DNA binding factors.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- The plusses and minuses of DNA torsion.eLife · 2025Article
- High-Resolution Genome-Wide Maps Reveal Widespread Presence of Torsional Insulation.bioRxiv : the preprint server for biology · 2025Article
- RSC and GRFs confer promoter directionality by restricting divergent noncoding transcription.Life science alliance · 2022Article
- Using time-lapse fluorescence microscopy to study gene regulation.Methods (San Diego, Calif.) · 2019Review
- Engineered bidirectional promoters enable rapid multi-gene co-expression optimization.Nature communications · 2018Article
- Systematic Study of Nucleosome-Displacing Factors in Budding Yeast.Molecular cell · 2018Article
- Gene regulation of mammalian long non-coding RNA.Molecular genetics and genomics : MGG · 2018Review
- Metagenomic Profiling of Soil Microbes to Mine Salt Stress Tolerance Genes.Frontiers in microbiology · 2018Article
- 3D clustering of co-regulated genes and its effect on gene expression.Current genetics · 2017Review
- Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeast.Nucleic acids research · 2017Article
- Paf1 Has Distinct Roles in Transcription Elongation and Differential Transcript Fate.Molecular cell · 2017Article
- Mediator binding to UASs is broadly uncoupled from transcription and cooperative with TFIID recruitment to promoters.The EMBO journal · 2016Article
- Timeless protection of telomeres.Current genetics · 2016Review
- The Determinants of Directionality in Transcriptional Initiation.Trends in genetics : TIG · 2016Review
- Regulation of cell-to-cell variability in divergent gene expression.Nature communications · 2016Article
- Characterization of Five Novel Brevibacillus Bacteriophages and Genomic Comparison of Brevibacillus Phages.PloS one · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Divergent gene pairs (DGPs) are abundant in eukaryotic genomes. Since two genes in a DGP potentially share the same regulatory sequence, one might expect that they should be co-regulated. However, an inspection of yeast DGPs containing cell-cycle or stress response genes revealed that most DGPs are differentially-regulated. The mechanism underlying DGP differential regulation is not understood. Here, we showed that co- versus differential regulation cannot be explained by genetic features including promoter length, binding site orientation, TATA elements, nucleosome distribution, or presence of non-coding RNAs. Using time-lapse fluorescence microscopy, we carried out an in-depth study of a differentially regulated DGP, PFK26-MOB1. We found that their differential regulation is mainly achieved through two DNA-binding factors, Tbf1 and Mcm1. Similar to 'enhancer-blocking insulators' in higher eukaryotes, these factors shield the proximal promoter from the action of more distant transcription regulators. We confirmed the blockage function of Tbf1 using synthetic promoters. We further presented evidence that the blockage mechanism is widely used among genome-wide DGPs. Besides elucidating the DGP regulatory mechanism, our work revealed a novel class of insulators in yeast.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.