ArticleInternational journal of molecular medicine2015
Grape pomace extract exerts antioxidant effects through an increase in GCS levels and GST activity in muscle and endothelial cells.
Article in International journal of molecular medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Rhizosphere microbiota adaptation under blueberry cultivation: modified paddy soil versus acidic red soil.Antonie van Leeuwenhoek · 2025Article
- Nutrients and Nutraceuticals fromNutrients · 2025Review
- Promising Phytogenic Feed Additives Used as Anti-Mycotoxin Solutions in Animal Nutrition.Toxins · 2024Review
- Grape Pomace Polyphenols as a Source of Compounds for Management of Oxidative Stress and Inflammation-A Possible Alternative for Non-Steroidal Anti-Inflammatory Drugs?Molecules (Basel, Switzerland) · 2022Review
- Reconceptualization of Hormetic Responses in the Frame of Redox Toxicology.International journal of molecular sciences · 2021Review
- Use of water and ethanol extracts from wine grape seed pomace to prepare an antioxidant toothpaste.Journal of the science of food and agriculture · 2021Article
- Research Advances in the Use of Bioactive Compounds fromAntioxidants (Basel, Switzerland) · 2020Review
- Grape seed proanthocyanidins improves mitochondrial function and reduces oxidative stress through an increase in sirtuin 3 expression in EA.hy926 cells in high glucose condition.Molecular biology reports · 2020Article
- Grape Polyphenols Ameliorate Muscle Decline Reducing Oxidative Stress and Oxidative Damage in Aged Rats.Nutrients · 2020Article
- In Vitro Toxicity Assessment of Stilbene Extract for Its Potential Use as Antioxidant in the Wine Industry.Antioxidants (Basel, Switzerland) · 2019Article
- Neuroprotective effects of Scrophularia buergeriana extract against glutamate-induced toxicity in SH-SY5Y cells.International journal of molecular medicine · 2019Article
- Effects of sheep/goat whey protein dietary supplementation on the redox status of rats.Molecular medicine reports · 2018Article
- Pterostilbene reduces oxidative stress, prevents hypertrophy and preserves systolic function of right ventricle in cor pulmonale model.British journal of pharmacology · 2017Article
- Fruits for Prevention and Treatment of Cardiovascular Diseases.Nutrients · 2017Review
- Natural Phenol Polymers: Recent Advances in Food and Health Applications.Antioxidants (Basel, Switzerland) · 2017Review
- Tissue specific effects of feeds supplemented with grape pomace or olive oil mill wastewater on detoxification enzymes in sheep.Toxicology reports · 2017Article
- Effect of arsenic acid withdrawal on hepatotoxicity and disruption of erythrocyte antioxidant defense system.Toxicology reports · 2017Article
- Oregano Essential Oil Induces SOD1 and GSH Expression through Nrf2 Activation and Alleviates Hydrogen Peroxide-Induced Oxidative Damage in IPEC-J2 Cells.Oxidative medicine and cellular longevity · 2016Article
- Polyphenolic composition of grape stem extracts affects antioxidant activity in endothelial and muscle cells.Molecular medicine reports · 2015Article
- Biofunctional Feed Supplemented With By-products of Olive Oil Production Improves Tissue Antioxidant Profile of Lambs.In vivo (Athens, Greece)Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In a previous study, we demonstrated that a grape pomace extract (GPE) exerted antioxidant activity in endothelial (EA.hy926) and muscle (C2C12) cells through an increase in glutathione (GSH) levels. In the present study, in order to elucidate the mechanisms responsible for the antioxidant activity of GPE, its effects on the expression of critical antioxidant enzymes, such as catalase (CAT), superoxide dismutase (SOD)1, heme oxygenase 1 (HO-1) and gamma-glutamylcysteine synthetase (GCS) were assessed in EA.hy926 and C2C12 cells. Moreover, the effects of GPE on CAT, SOD and glutathione S-transferase (GST) enzymatic activity were evaluated. For this purpose, the C2C12 and EA.hy926 cells were treated with GPE at low and non-cytotoxic concentrations (2.5 and 10 µg/ml for the C2C12 cells; 0.068 and 0.250 µg/ml for the EA.hy926 cells) for 3, 6, 12, 18 and 24 h. Following incubation, enzymatic expression and activity were assessed. The results revealed that treatment with GPE significantly increased GCS levels and GST activity in both the C2C12 and EA.hy926 cells. However, GPE significantly decreased CAT levels and activity, but only in the muscle cells, while it had no effect on CAT levels and activity in the endothelial cells. Moreover, treatment with GPE had no effect on HO-1 and SOD expression and activity in both cell lines. Therefore, the present results provide further evidence of the crucial role of GSH systems in the antioxidant effects exerted by GPE. Thus, GPE may prove to be effective for use as a food supplement for the treatment of oxidative stress-induced pathological conditions of the cardiovascular and skeletal muscle systems, particularly those associated with low GSH levels.
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