ArticleJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism2015
Optogenetic stimulation of GABA neurons can decrease local neuronal activity while increasing cortical blood flow.
Article in Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers, 1 of them a synthesis that pooled it.
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Who cites it
82 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Optogenetic fMRI for Brain-Wide Circuit Analysis of Sensory Processing.International journal of molecular sciences · 2022Pooled it
- Negative BOLD response to interictal discharges in epilepsy using intracranial EEG-fMRI.Neuroimage. Reports · 2026Article
- Simultaneous single-cell calcium imaging of neuronal population activity and brain-wide BOLD fMRI.Nature methods · 2026Article
- Parvalbumin interneurons contribute to spontaneous hemodynamic fluctuations.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2026Article
- Resting fMRI functional connectivity reflects fluctuations in inhibitory interneuron activity.bioRxiv : the preprint server for biology · 2026Article
- BOLD signal changes can oppose oxygen metabolism across the human cortex.Nature neuroscience · 2026Article
- Orbitofrontal PV interneurons modulate social interaction via default mode network dynamics.Communications biology · 2026Article
- BOLD response delays represent local cortical processing.Cerebral cortex (New York, N.Y. : 1991) · 2026Article
- Neural mechanisms underlying intracortical microstimulation for sensory restoration.Nature biomedical engineering · 2026Review
- Article
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- Nitric oxide is not responsible for initial sensory-induced neurovascular coupling response in the barrel cortex of lightly anesthetized mice.Neurophotonics · 2025Article
- Correlation of zero echo time functional MRI with neuronal activity in rats.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2025Article
- Neuromodulation in Small Animal fMRI.Journal of magnetic resonance imaging : JMRI · 2025Review
- Neural circuit mechanisms underlying aberrantly prolonged functional hyperemia in young Alzheimer's disease mice.Molecular psychiatry · 2025Article
- Revealing excitation-inhibition imbalance in Alzheimer's disease using multiscale neural model inversion of resting-state functional MRI.Communications medicine · 2025Article
- Cerebral blood flow patterns induced by photoactivation based on laser speckle contrast imaging.Biomedical optics express · 2024Article
- Serotonergic regulation of cortical neurovascular coupling and hemodynamics upon awakening from sleep in mice.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2024Article
- Parvalbumin interneuron activity induces slow cerebrovascular fluctuations in awake mice.bioRxiv : the preprint server for biology · 2024Article
- Distinct neurochemical influences on fMRI response polarity in the striatum.Nature communications · 2024Article
22 more citing papers are in PubMed but not listed here.
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5 authors.
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Abstract
We investigated the link between direct activation of inhibitory neurons, local neuronal activity, and hemodynamics. Direct optogenetic cortical stimulation in the sensorimotor cortex of transgenic mice expressing Channelrhodopsin-2 in GABAergic neurons (VGAT-ChR2) greatly attenuated spontaneous cortical spikes, but was sufficient to increase blood flow as measured with laser speckle contrast imaging. To determine whether the observed optogenetically evoked gamma aminobutyric acid (GABA)-neuron hemodynamic responses were dependent on ionotropic glutamatergic or GABAergic synaptic mechanisms, we paired optogenetic stimulation with application of antagonists to the cortex. Incubation of glutamatergic antagonists directly on the cortex (NBQX and MK-801) blocked cortical sensory evoked responses (as measured with electroencephalography and intrinsic optical signal imaging), but did not significantly attenuate optogenetically evoked hemodynamic responses. Significant light-evoked hemodynamic responses were still present after the addition of picrotoxin (GABA-A receptor antagonist) in the presence of the glutamatergic synaptic blockade. This activation of cortical inhibitory interneurons can mediate large changes in blood flow in a manner that is by and large not dependent on ionotropic glutamatergic or GABAergic synaptic transmission. This supports the hypothesis that activation of inhibitory neurons can increase local cerebral blood flow in a manner that is not entirely dependent on levels of net ongoing neuronal activity.
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