ArticleScientific reports2015
Improving access to endogenous DNA in ancient bones and teeth.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 127 papers.
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127 citing papers in PubMed, 273 citations in OpenAlex.
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- Jomon genomics reveal cold adaptation in Upper Paleolithic hunter-gatherers of eastern Eurasia.Science advances · 2026Article
- Development and Validation of PCR-Based Ancient DNA Analysis for Italian and Western Balkan Archaeological Collections.Balkan journal of medical genetics : BJMG · 2026Article
- The First Ancient DNA Evidence of Zebu Husbandry in Thailand During the Prehistoric Through the Historic Periods.Animals : an open access journal from MDPI · 2026Article
- Mesopotamian ancient DNA reveals Iron Age integration of heterogeneous Bronze Age genetic ancestries following resettlement.Genome biology · 2026Article
- Ancient DNA from the Upper Paleolithic mammoth ivory of Hohle Fels, Germany.Scientific reports · 2026Article
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- A large mass grave from the Early Iron Age indicates selective violence towards women and children in the Carpathian Basin.Nature human behaviour · 2026Article
- Population discontinuity in the Paris Basin linked to evidence of the Neolithic decline.Nature ecology & evolution · 2026Article
- Ancient DNA studies performed in the forensic genetic laboratory in Slovenia: a narrative review.Croatian medical journal · 2026Review
- The genomic history of Streptococcus mutans from the Mesolithic until modern times.Genome biology · 2026Article
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- Tracing 2500 years of human betaherpesvirus 6A and 6B diversity through ancient DNA.Science advances · 2026Article
- Demographic history of the Jomon people: insights from whole-mitogenome analysis.Anthropological science : journal of the Anthropological Society of Nippon = Jinruigaku zasshi · 2026Article
- Identification and removal of contamination in palaeoproteomic analysis of dental enamel.Scientific reports · 2025Article
- Lessons learned: Recommendations for reproducible paleogenomic data analyses.American journal of human genetics · 2025Review
- Ancient DNA reveals the population interactions and a Neolithic patrilineal community in Northern Yangtze Region.Nature communications · 2025Article
- Bone Adhered Sediments as a Source of Target and Environmental DNA and Proteins.Molecular biology and evolution · 2025Article
67 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Poor DNA preservation is the most limiting factor in ancient genomic research. In the majority of ancient bones and teeth, endogenous DNA molecules represent a minor fraction of the whole DNA extract, rendering shot-gun sequencing inefficient for obtaining genomic data. Based on ancient human bone samples from temperate and tropical environments, we show that an EDTA-based enzymatic 'pre-digestion' of powdered bone increases the proportion of endogenous DNA several fold. By performing the pre-digestion step between 30 min and 6 hours on five bones, we observe an asymptotic increase in endogenous DNA content, with a 2.7-fold average increase reached at 1 hour. We repeat the experiment using a brief pre-digestion (15 or 30 mins) on 21 ancient bones and teeth from a variety of archaeological contexts and observe an improvement in 16 of these. We here advocate the implementation of a brief pre-digestion step as a standard procedure in ancient DNA extractions. Finally, we demonstrate on 14 ancient teeth that by targeting the outer layer of the roots we obtain up to 14 times more endogenous DNA than when using the inner dentine. Our presented methods are likely to increase the proportion of ancient samples that are suitable for genome-scale characterization.
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