ArticleScientific reports2015
Visualizing the activity of Escherichia coli divergent promoters and probing their dependence on superhelical density using dual-colour fluorescent reporter vector.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
- Analysis of Teg41 and PSMα promoter activity using a divergent fluorescent reporter plasmid.mSphere · 2025Article
- From DNA to lytic proteins: transcription and translation of the bacteriophage T5 holin/endolysin operon.World journal of microbiology & biotechnology · 2024Article
- Standardization of regulatory nodes for engineering heterologous gene expression: a feasibility study.Microbial biotechnology · 2022Article
- Analytical kinetic model of native tandem promoters in E. coli.PLoS computational biology · 2022Article
- Divergently Transcribed ncRNAs inFrontiers in molecular biosciences · 2021Article
- Excessive Promoters as Silencers of Genes Horizontally Acquired byFrontiers in molecular biosciences · 2020Article
- Engineered bidirectional promoters enable rapid multi-gene co-expression optimization.Nature communications · 2018Article
- The nucleoid protein Dps binds genomic DNA of Escherichia coli in a non-random manner.PloS one · 2017Article
- Inter-sigmulon communication through topological promoter coupling.Nucleic acids research · 2016Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mosaic pattern of transcription in alternating directions is a common feature of prokaryotic and eukaryotic genomes which rationality and origin remain enigmatic. In Escherichia coli approximately 25% of genes comprise pairs of topologically linked divergently transcribed units. Given that transcriptional complex formation at each promoter in the pair induces topological changes and is itself sensitive to DNA structural perturbations, study of the functional anatomy in such areas requires special approaches. Here we suggested the dual-colour promoter probe vector which may become an ideal tool for divergent transcription profiling. The vector was used to characterize the specific genomic region nearby appY with multiple bidirectional promoters predicted in silico. Only three promoters of this region were shown to be engaged in the transcription initiation resulting in the expression of reporter genes. RNA product transcribed in antisense direction is suggested as a novel RNA. Nalidixin-induced topological modulation differentially affected transcription in sense and antisense directions thus exemplifying anticooperative mode in the response to topological alterations.
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