ArticleWorld journal of microbiology & biotechnology2015
Comparison of ITS and 18S rDNA for estimating fungal diversity using PCR-DGGE.
Article in World journal of microbiology & biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Neglected kingdoms: the gut virome, mycobiome and their role in inflammatory bowel disease.Gut microbes · 2026Review
- Culture-media-based evaluation of fungal diversity in petroleum-produced water: assessment of extracellular enzyme production.Biologia futura · 2026Article
- Uncovering the Community Structure and Adaptive Characteristics of Soil Protists Across Natural, Semi-Natural and Highly Degraded Habitats.Environmental microbiology · 2026Article
- Phyllosphere microbiomes uncovered: Research trends, geographic disparities, and key microbial players.Genetics and molecular biology · 2026Article
- Saline-alkali resilience: the role of Trametes NF1 in promoting alfalfa growth and salinity tolerance.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Biological Activities of Aspergillus niger MF6: Antimicrobial, Antioxidant, and Cytotoxic Potential of Marine-Derived Fungus Metabolites.Current microbiology · 2025Article
- Overview of Microorganisms: Bacterial Microbiome, Mycobiome, Virome Identified Using Next-Generation Sequencing, and Their Application to Ophthalmic Diseases.Microorganisms · 2025Review
- Multi-marker comparative analysis of 18S, ITS1, and ITS2 primers for human gut mycobiome profiling.Frontiers in bioinformatics · 2025Article
- Identification of potential insect ecological interactions using a metabarcoding approach.PeerJ · 2025Article
- Geographic Distribution Pattern Determines Soil Microbial Community Assembly Process inMicroorganisms · 2024Article
- Gut Mycobiome and Asthma.Journal of fungi (Basel, Switzerland) · 2024Review
- Assessment of DNA extraction methods for human gut mycobiome analysis.Royal Society open science · 2024Article
- Article
- Screening of endophytic fungi from Cremastra appendiculata and their potential for plant growth promotion and biological control.Folia microbiologica · 2023Article
- Article
- Revealing the secret life of skin - with the microbiome you never walk alone.International journal of cosmetic science · 2020Review
- MALDI-TOF MS for the rapid identification and drug susceptibility testing of filamentous fungi.Experimental and therapeutic medicine · 2019Article
- Article
- Influence of a Serratia marcescens outbreak on the gut microbiota establishment process in low-weight preterm neonates.PloS one · 2019Article
- A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms.BMC microbiology · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Both the internal transcribed spacer (ITS) region and 18S rRNA genes are broadly applied in molecular fingerprinting studies of fungi. However, the differences in those two ribosomal RNA regions are still largely unknown. In the current study, three sets of most suitable subunit ribosomes in ITS and 18S rRNA were compared using denaturing gradient gel electrophoresis (DGGE) under the optimum experimental conditions. Ten samples from both aquatic and soil environments were tested. The results revealed that the ITS region produced range-weighted richness in the range 36-361, which was significantly higher than that produced by 18S rDNA. There was a similar tendency in terms of the Shannon-Weaver diversity index and community dynamics in both water and soil samples. Samples from water and soil were better separated using ITS than 18S rDNA in principal component analysis of DGGE bands. Our study suggests that the ITS region is more precise and has more potential than 18S rRNA genes in fungal community analysis.
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