ArticlePloS one2015
Evaluating the Species Boundaries of Green Microalgae (Coccomyxa, Trebouxiophyceae, Chlorophyta) Using Integrative Taxonomy and DNA Barcoding with Further Implications for the Species Identification in Environmental Samples.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- First records of cyanobacteria and microalgae in sand dunes of the Russian high north: morphological and DNA barcoding evidence.Archives of microbiology · 2026Article
- Guidance on the characterisation of microorganisms in support of the risk assessment of products used in the food chain.EFSA journal. European Food Safety Authority · 2025Article
- The Geosiphon-Nostoc symbiosis: recent elaboration or remnant of an enduring association?Annals of botany · 2025Article
- High quality genome assembly and annotation (v1) of the eukaryotic freshwater microalga Coccomyxa elongata SAG 216-3b.G3 (Bethesda, Md.) · 2025Article
- Phylogeny and fatty acid profiles of newFrontiers in microbiology · 2025Article
- The chloroplast genome of theFrontiers in genetics · 2025Article
- Developing Strain-Specific Simple Sequence Repeat (SSR) Markers forJournal of microbiology and biotechnology · 2024Article
- Terrestrial mosses as a substrate and potential host for cyanobacteria, green algae and diatoms.Journal of plant research · 2024Article
- Review
- Alcobiosis, an algal-fungal association on the threshold of lichenisation.Scientific reports · 2023Article
- Metabolite Profiling in Green Microalgae with Varying Degrees of Desiccation Tolerance.Microorganisms · 2022Article
- Nutritional conditions of the novel freshwater Coccomyxa AP01 for versatile fatty acids composition.Journal of applied microbiology · 2022Article
- Molecular diet analysis enables detection of diatom and cyanobacteria DNA in the gut of Macoma balthica.PloS one · 2022Article
- Unexpected cryptic species among streptophyte algae most distant to land plants.Proceedings. Biological sciences · 2021Article
- Review
- Metabolomics and lipid profile analysis of Coccomyxa melkonianii SCCA 048.Extremophiles : life under extreme conditions · 2021Article
- Genetic Diversity of Symbiotic Green Algae ofPlants (Basel, Switzerland) · 2021Article
- Selection and re-acclimation of bioprospected acid-tolerant green microalgae suitable for growth at low pH.Extremophiles : life under extreme conditions · 2021Article
- Identification of microalgae cultured in Bold's Basal medium from freshwater samples, from a high-rise city.Scientific reports · 2021Article
- Uncovering Unique Green Algae and Cyanobacteria Isolated from Biocrusts in Highly Saline Potash Tailing Pile Habitats, Using an Integrative Approach.Microorganisms · 2020Article
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5 authors.
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Abstract
Integrative taxonomy is an approach for defining species and genera by taking phylogenetic, morphological, physiological, and ecological data into account. This approach is appropriate for microalgae, where morphological convergence and high levels of morphological plasticity complicate the application of the traditional classification. Although DNA barcode markers are well-established for animals, fungi, and higher plants, there is an ongoing discussion about suitable markers for microalgae and protists because these organisms are genetically more diverse compared to the former groups. To solve these problems, we assess the usage of a polyphasic approach combining phenotypic and genetic parameters for species and generic characterization. The application of barcode markers for database queries further allows conclusions about the 'coverage' of culture-based approaches in biodiversity studies and integrates additional aspects into modern taxonomic concepts. Although the culture-dependent approach revealed three new lineages, which are described as new species in this paper, the culture-independent analyses discovered additional putative new species. We evaluated three barcode markers (V4, V9 and ITS-2 regions, nuclear ribosomal operon) and studied the morphological and physiological plasticity of Coccomyxa, which became a model organism because its whole genome sequence has been published. In addition, several biotechnological patents have been registered for Coccomyxa. Coccomyxa representatives are distributed worldwide, are free-living or in symbioses, and colonize terrestrial and aquatic habitats. We investigated more than 40 strains and reviewed the biodiversity and biogeographical distribution of Coccomyxa species using DNA barcoding. The genus Coccomyxa formed a monophyletic group within the Trebouxiophyceae separated into seven independent phylogenetic lineages representing species. Summarizing, the combination of different characteristics in an integrative approach helps to evaluate environmental data and clearly identifies microalgae at generic and species levels.
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