Evidence map›Paper›PMID 26079703›Full record

ArticleAnalytical biochemistry2015

Evaluation of the specificity of four commercially available antibodies to alpha-syntrophin.

Kristina Eisinger, Stanley C Froehner, Marvin E Adams, Sabrina Krautbauer, Christa Buechler

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Kristina EisingerDepartment of Internal Medicine I, University Hospital of Regensburg, 93042 Regensburg, Germany.
Stanley C FroehnerDepartment of Physiology and Biophysics, University of Washington, WA, USA.
Marvin E AdamsDepartment of Physiology and Biophysics, University of Washington, WA, USA.
Sabrina KrautbauerDepartment of Internal Medicine I, University Hospital of Regensburg, 93042 Regensburg, Germany.
Christa BuechlerDepartment of Internal Medicine I, University Hospital of Regensburg, 93042 Regensburg, Germany. Electronic address: christa.buechler@klinik.uni-regensburg.de.
University Hospital Regensburg · DEUniversity of Washington · US

Funding

Regulation of Syntrophin FunctionR01NS033145 · NINDS · UNIVERSITY OF WASHINGTON · PI FROEHNER, STANLEY C · 1995 to 2013
$5.2M
NINDS NIH HHS R01 NS033145NINDS NIH HHS R01NS33145
6 · The paper itself

Abstract

Alpha-syntrophin (SNTA) is an adaptor protein that regulates several signaling pathways. To analyze expression of SNTA immunoblot assays must be performed. Here, the specificity of four commercially available SNTA antibodies has been evaluated in immunoblot experiments using liver tissues of wild-type and SNTA-deficient mice. While one of the antibodies reacts with SNTA, two antibodies specifically recognize beta 2 syntrophin (SNTB2). The antigen detected by the fourth antibody has not been identified but is different from SNTA and SNTB2. Therefore, only one of the four tested antibodies is appropriate to analyze SNTA protein levels by immunoblot.

Indexed as

Antibody SpecificityImmunoblottingAnimalsCalcium-Binding ProteinsLiverMembrane ProteinsMiceMuscle ProteinsCalcium-Binding ProteinsMembrane ProteinsMuscle Proteinssyntrophin alpha1ImmunoblotKnockout miceLiver

Identifiers

PMID26079703
PMCPMC4497918
OpenAlexW609880476

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.