ArticleScientific reports2015
Visualizing the appearance and disappearance of the attractor of differentiation using Raman spectral imaging.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
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- Extracellular vesicles may provide an alternative detoxification pathway during skeletal muscle myoblast ageing.Journal of extracellular biology · 2024Article
- Yuragi biomarker concept for evaluating human induced pluripotent stem cells using heterogeneity-based Raman finger-printing.Biophysics and physicobiology · 2024Article
- Review
- Application of the Dynamical Network Biomarker Theory to Raman Spectra.Biomolecules · 2022Article
- Recent Advances in Raman Spectral Imaging in Cell Diagnosis and Gene Expression Prediction.Genes · 2022Review
- Coherent anti-Stokes Raman scattering cell imaging and segmentation with unsupervised data analysis.Frontiers in cell and developmental biology · 2022Article
- Label-free classification of neurons and glia in neural stem cell cultures using a hyperspectral imaging microscopy combined with machine learning.Scientific reports · 2019Article
- An Expandable Mechanopharmaceutical Device (3): a Versatile Raman Spectral Cytometry Approach to Study the Drug Cargo Capacity of Individual Macrophages.Pharmaceutical research · 2018Article
- Classification of C2C12 cells at differentiation by convolutional neural network of deep learning using phase contrast images.Human cell · 2018Article
- Bright Dots and Smart Optical Microscopy to Probe Intracellular Events in Single Cells.Frontiers in bioengineering and biotechnology · 2018Review
- Protein expression guided chemical profiling of living cells by the simultaneous observation of Raman scattering and anti-Stokes fluorescence emission.Scientific reports · 2017Article
- Non-label immune cell state prediction using Raman spectroscopy.Scientific reports · 2016Article
- In situ visualization of intracellular morphology of epidermal cells using stimulated Raman scattering microscopy.Journal of biomedical optics · 2016Article
- Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it.Scientific reports · 2016Article
- Spectroscopic profiling variations in extracellular vesicle biochemistry in a model of myogenesis.Journal of tissue engineeringArticle
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Authors and funding
7 authors.
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Abstract
Using Raman spectral imaging, we visualized the cell state transition during differentiation and constructed hypothetical potential landscapes for attractors of cellular states on a state space composed of parameters related to the shape of the Raman spectra. As models of differentiation, we used the myogenic C2C12 cell line and mouse embryonic stem cells. Raman spectral imaging can validate the amounts and locations of multiple cellular components that describe the cell state such as proteins, nucleic acids, and lipids; thus, it can report the state of a single cell. Herein, we visualized the cell state transition during differentiation using Raman spectral imaging of cell nuclei in combination with principal component analysis. During differentiation, cell populations with a seemingly homogeneous cell state before differentiation showed heterogeneity at the early stage of differentiation. At later differentiation stages, the cells returned to a homogeneous cell state that was different from the undifferentiated state. Thus, Raman spectral imaging enables us to illustrate the disappearance and reappearance of an attractor in a differentiation landscape, where cells stochastically fluctuate between states at the early stage of differentiation.
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