ArticlePhysiology & behavior2015
Mouse handling limits the impact of stress on metabolic endpoints.
Article in Physiology & behavior, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers, 2 of them syntheses that pooled it.
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Who cites it
65 citing papers in PubMed, 2 syntheses or guidelines pooled it, 114 citations in OpenAlex.
- A systematic review of the development and application of home cage monitoring in laboratory mice and rats.BMC biology · 2023Pooled it
- FELASA recommendations for the rehoming of animals used for scientific and educational purposes.Laboratory animals · 2023Guideline
- Prefrontal-medullary circuitry is necessary for sex-specific responses to metabolic stress in rats.Endocrinology · 2026Article
- Circadian Reprogramming by Combined Time-Restricted Feeding and Exercise Improves Metabolic Homeostasis in Diabetes.Metabolites · 2026Article
- Enhancing Rodent Welfare, Cost Savings, and Efficiency: Implementation and Review of Data-Driven Improvements.Journal of the American Association for Laboratory Animal Science : JAALAS · 2026Review
- Prefrontal-medullary circuitry is necessary for sex-specific responses to metabolic stress in rats.bioRxiv : the preprint server for biology · 2026Article
- Effects of Scruff Restraint and Handling-Tail and Tunnel-on Plasma Corticosterone in Female BALB/c Mice (Mus musculus).Journal of the American Association for Laboratory Animal Science : JAALAS · 2026Article
- Article
- Robust noninvasive detection of hyperglycemia in mouse models of metabolic dysregulation using the novel Urination Index biomarker.Lab animal · 2025Article
- Strategies for optimization of hypoglycemia rat models.Animal models and experimental medicine · 2025Review
- Inhibition of Semaphorin 3A in Hippocampus Alleviates Postpartum Depression-Like Behaviors in Mice.Molecular neurobiology · 2025Article
- Low-dose quinine targets KCNH6 to potentiate glucose-induced insulin secretion.Journal of molecular cell biology · 2025Article
- Revisited guidelines for metabolic tolerance tests in mice.Lab animal · 2025Review
- The effects of handling on mouse behavior: cupped hands versus familiar or novel huts or tunnels.PloS one · 2025Article
- The implementation of tunnel handling in a mouse breeding facility revealed strain-specific behavioural responses.Laboratory animals · 2024Article
- Glucagon receptor activation contributes to the development of kidney injury.American journal of physiology. Renal physiology · 2024Article
- Metabolic and Skeletal Characterization of the KK/ACalcified tissue international · 2024Article
- Automated home cage monitoring of an aging colony of mice-Implications for welfare monitoring and experimentation.Frontiers in neuroscience · 2024Article
- Social isolation leads to mild social recognition impairment and losses in brain cellularity.Brain structure & function · 2023Article
- Continuous deep brain stimulation of the nucleus accumbens reduces food intake but does not affect body weight in mice fed a high-fat diet.Scientific reports · 2023Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Studies focused on end-points that are confounded by stress are best performed under minimally stressful conditions. The objective of this study was to demonstrate the impact of handling designed to reduce animal stress on measurements of glucose tolerance. A cohort of mice (CD1.C57BL/6) naïve to any specific handling was subjected to either a previously described "cup" handling method, or a "tail-picked" method in which the animals were picked up by the tail (as is common for metabolic studies). Following training, an elevated plus maze (EPM) test was performed followed by measurement of blood glucose and plasma corticosterone. A second cohort (CD1.C57BL/6) was rendered obese by exposure to a high fat diet, handled with either the tail-picked or cup method and subjected to an intraperitoneal glucose tolerance test. A third cohort of C57BL/6 mice was exposed to a cup regimen that included a component of massage and was subjected to tests of anxiety-like behavior, glucose homeostasis, and corticosterone secretion. We found that the cup mice showed reduced anxiety-like behaviors in the EPM coupled with a reduction in blood glucose levels compared to mice handled by the tail-picked method. Additionally, cup mice on the high fat diet exhibited improved glucose tolerance compared to tail-picked controls. Finally, we found that the cup/massage group showed lower glucose levels following an overnight fast, and decreased anxiety-like behaviors associated with lower stress-induced plasma corticosterone concentration compared to tail-picked controls. These data demonstrate that application of handling methods that reduce anxiety-like behaviors in mice mitigates the confounding contribution of stress to interpretation of metabolic endpoints (such as glucose tolerance).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.