ArticlePlant biotechnology journal2016
Identification of low Ca(2+) stress-induced embryo apoptosis response genes in Arachis hypogaea by SSH-associated library lift (SSHaLL).
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 22 citations in OpenAlex.
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- Molecular Cloning and Functional Identification of a Pericarp- and Testa-Abundant Gene's (International journal of molecular sciences · 2024Article
- A novel approach: enhancing marigold (Frontiers in plant science · 2024Article
- Molecular cloning and functional characterization of the promoter of a novelFrontiers in plant science · 2023Article
- The genusFrontiers in plant science · 2023Review
- The root-specific NtR12 promoter-based expression of RIP increased the resistance against bacterial wilt disease in tobacco.Molecular biology reports · 2022Article
- Identification of Key Gene Networks and Deciphering Transcriptional Regulators Associated With Peanut Embryo Abortion Mediated by Calcium Deficiency.Frontiers in plant science · 2022Article
- Cloning and Functional Characterization of a Pericarp Abundant Expression Promoter (Frontiers in genetics · 2021Article
- High-density SNP map facilitates fine mapping of QTLs and candidate genes discovery for Aspergillus flavus resistance in peanut (Arachis hypogaea).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Ectopic Expression ofGenes · 2020Article
- Overexpression of the peanut CLAVATA1-like leucine-rich repeat receptor-like kinase AhRLK1 confers increased resistance to bacterial wilt in tobacco.Journal of experimental botany · 2019Article
- Integrated microRNA and transcriptome profiling reveals a miRNA-mediated regulatory network of embryo abortion under calcium deficiency in peanut (Arachis hypogaea L.).BMC genomics · 2019Article
- Peg Biology: Deciphering the Molecular Regulations Involved During Peanut Peg Development.Frontiers in plant science · 2019Review
- Article
- Transcriptome and Differential Expression Profiling Analysis of the Mechanism of CaFrontiers in plant science · 2017Article
- Overexpression of a novel peanut NBS-LRR gene AhRRS5 enhances disease resistance to Ralstonia solanacearum in tobacco.Plant biotechnology journal · 2017Article
- Characterization of NtREL1, a novel root-specific gene from tobacco, and upstream promoter activity analysis in homologous and heterologous hosts.Plant cell reports · 2016Article
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calcium is a universal signal in the regulation of wide aspects in biology, but few are known about the function of calcium in the control of early embryo development. Ca(2+) deficiency in soil induces early embryo abortion in peanut, producing empty pods, which is a general problem; however, the underlying mechanism remains unclear. In this study, embryo abortion was characterized to be caused by apoptosis marked with cell wall degradation. Using a method of SSH cDNA libraries associated with library lift (SSHaLL), 62 differentially expressed genes were isolated from young peanut embryos. These genes were classified to be stress responses, catabolic process, carbohydrate and lipid metabolism, embryo morphogenesis, regulation, etc. The cell retardation with cell wall degradation was caused by up-regulated cell wall hydrolases and down-regulated cellular synthases genes. HsfA4a, which was characterized to be important to embryo development, was significantly down-regulated under Ca(2+) -deficient conditions from 15 days after pegging (DAP) to 30 DAP. Two AhCYP707A4 genes, encoding abscisic acid (ABA) 8'-hydroxylases, key enzymes for ABA catabolism, were up-regulated by 21-fold under Ca(2+) -deficient conditions upstream of HsfA4a, reducing the ABA level in early embryos. Over-expression of AhCYP707A4 in Nicotiana benthamiana showed a phenotype of low ABA content with high numbers of aborted embryos, small pods and less seeds, which confirms that AhCYP707A4 is a key player in regulation of Ca(2+) deficiency-induced embryo abortion via ABA-mediated apoptosis. The results elucidated the mechanism of low Ca(2+) -induced embryo abortion and described the method for other fields of study.
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