ArticleBioMed research international2015
Sensitive Detection of Thirteen Bacterial Vaginosis-Associated Agents Using Multiplex Polymerase Chain Reaction.
Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- Efficacy and Safety of MED-01 Probiotics on Vaginal Health: A 12-Week, Multicenter, Randomized, Double-Blind, Placebo-Controlled Clinical Trial.Nutrients · 2023Trial
- Comparative Performance of Aptima BV/CV-TV and Vaginal Real Time Assays for the Molecular Diagnosis of Bacterial Vaginosis and Vaginitis.Diagnostics (Basel, Switzerland) · 2026Article
- Long-Term Reproductive Outcomes After Palmer-Type Neosalpingostomy in Hydrosalpinx: A Seven-Year Real-World Cohort Study.Journal of clinical medicine · 2025Article
- Molecular characterization of vaginal microbiota using a new 22-species qRT-PCR test to achieve a relative-abundance and species-based diagnosis of bacterial vaginosis.Frontiers in cellular and infection microbiology · 2024Article
- The associations between low abundance of Mycoplasma hominis and female fecundability: a pregnancy-planning cohort study.BMC microbiology · 2022Article
- BV associated bacteria specifically BVAB 1 and BVAB 3 as biomarkers for HPV risk and progression of cervical neoplasia.Infectious diseases in obstetrics and gynecology · 2022Article
- Molecular Detection of Sexually Transmitted Infections in Women with and without Human Papillomaviruses Infection Who Referred to Tehran West Hospitals in Iran.Reports of biochemistry & molecular biology · 2021Article
- Strong correlation between urine and vaginal swab samples for bacterial vaginosis.Southern African journal of infectious diseases · 2021Article
- Lack of association between Mycoplasma hominis and Trichomonas vaginalis symbiosis in relation to metronidazole resistance.Parasitology research · 2020Article
- Vaginal microbiota evaluation and prevalence of key pathogens in ecuadorian women: an epidemiologic analysis.Scientific reports · 2020Article
- Predictive value of cervical cytokine, antimicrobial and microflora levels for pre-term birth in high-risk women.Scientific reports · 2019Article
- Male Partners of Infertile Couples With Seminal Positivity for Markers of Bacterial Vaginosis Have Impaired Fertility.American journal of men's health · 2018Article
- Length-Heterogeneity Polymerase Chain Reaction as a Diagnostic Tool for Bacterial Vaginosis.Chinese medical journal · 2018Article
- Syndromic Diagnosis in Evaluation of Women with Symptoms of Vaginitis.Current infectious disease reports · 2017Review
- Ureaplasma parvum genotype, combined vaginal colonisation with Candida albicans, and spontaneous preterm birth in an Australian cohort of pregnant women.BMC pregnancy and childbirth · 2016Article
- Association of human papillomavirus, Neisseria gonorrhoeae and Chlamydia trachomatis co-infections on the risk of high-grade squamous intraepithelial cervical lesion.American journal of cancer research · 2016Article
- Increased Risk of Infertility in Women Infected with Human Papillomavirus.Journal of reproduction & infertilityArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial vaginosis (BV) is characterized by a polymicrobial proliferation of anaerobic bacteria and depletion of lactobacilli, which are components of natural vaginal microbiota. Currently, there are limited conventional methods for BV diagnosis, and these methods are time-consuming, expensive, and rarely allow for the detection of more than one agent simultaneously. Therefore, we conceived and validated a multiplex polymerase chain reaction (M-PCR) assay for the simultaneous screening of thirteen bacterial vaginosis-associated agents (BV-AAs) related to symptomatic BV: Gardnerella vaginalis, Mobiluncus curtisii, Mobiluncus mulieris, Bacteroides fragilis, Mycoplasma hominis, Atopobium vaginae, Ureaplasma urealyticum, Megasphaera type I, Clostridia-like bacteria vaginosis-associated bacteria (BVABs) 1, 2, and 3, Sneathia sanguinegens, and Mycoplasma genitalium. The overall validation parameters of M-PCR compared to single PCR (sPCR) were extremely high, including agreement of 99.1% and sensitivity, specificity, and positive predictive values of 100.0%, negative predictive value of 97.0%, accuracy of 99.3%, and agreement with Nugent results of 100.0%. The prevalence of BV-AAs was very high (72.6%), and simultaneous agents were detected in 53.0%, which demonstrates the effectiveness of the M-PCR assay. Therefore, the M-PCR assay has great potential to impact BV diagnostic methods in vaginal samples and diminish associated complications in the near future.
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