Evidence map›Paper›PMID 26078915›Full record

ArticleJournal of integrated OMICS2013

Revisiting Protocols for the NMR Analysis of Bacterial Metabolomes.

Steven Halouska, Bo Zhang, Rosmarie Gaupp, Shulei Lei, Emily Snell, Robert J Fenton, Raul G Barletta, Greg A Somerville, Robert Powers

Abstract read
In one paragraph

Article in Journal of integrated OMICS, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. The Development and Validation of Radiopharmaceuticals Targeting Bacterial Infection.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2023
    Article
  5. Sample Preparation in Microbial Metabolomics: Advances and Challenges.Advances in experimental medicine and biology · 2023
    Article
  6. Advances in Microbial NMR Metabolomics.Advances in experimental medicine and biology · 2023
    Article
  7. Bacterial Metabolomics: Sample Preparation Methods.Biochemistry research international · 2022
    Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Metabolomics Analyses from Tissues in Parkinson's Disease.Methods in molecular biology (Clifton, N.J.) · 2019
    Article
  16. NMR Metabolomics Protocols for Drug Discovery.Methods in molecular biology (Clifton, N.J.) · 2019
    Article
  17. Article
  18. Review
  19. Comparing normalization methods and the impact of noise.Metabolomics : Official journal of the Metabolomic Society · 2018
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Steven HalouskaDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304.
Bo ZhangDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304.
Rosmarie GauppSchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-0905.
Shulei LeiDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304.
Emily SnellDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304.
Robert J FentonSchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-0905.
Raul G BarlettaSchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-0905.
Greg A SomervilleSchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-0905.
Robert PowersDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304.

Funding

THE REDOX-MEDIATED REGULATION OF IRON-RESPONSIVE GENE EXPRESSION IN LIVERP20RR017675 · NCRR · UNIVERSITY OF NEBRASKA LINCOLN · PI GRASS, GREGOR · 2002 to 2011
$20.4M
Redox Biology CenterP30GM103335 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI BECKER, DONALD F · 2012 to 2016
$4.3M
EXTRAMURAL RESEARCH FACILITIES CONSTRUCTIONC06RR015468 · NCRR · UNIVERSITY OF NEBRASKA LINCOLN · PI PAUL, PREM S · 2000 to 2000
$2.0M
Citric acid cycle regulation of exopolysaccharide synthesis in staphylococciR01AI087668 · NIAID · UNIVERSITY OF NEBRASKA LINCOLN · PI SOMERVILLE, GREG ALAN · 2010 to 2013
$1.4M
Essentiality of Mycobacterium tuberculosis D-alanine racemaseR21AI087561 · NIAID · UNIVERSITY OF NEBRASKA LINCOLN · PI CHACON, OFELIA · 2010 to 2011
$393k
NCRR NIH HHS C06 RR015468NCRR NIH HHS P20 RR017675NIAID NIH HHS R01 AI087668NIAID NIH HHS R21 AI087561NIGMS NIH HHS P30 GM103335
6 · The paper itself

Abstract

Over the past decade, metabolomics has emerged as an important technique for systems biology. Measuring all the metabolites in a biological system provides an invaluable source of information to explore various cellular processes, and to investigate the impact of environmental factors and genetic modifications. Nuclear magnetic resonance (NMR) spectroscopy is an important method routinely employed in metabolomics. NMR provides comprehensive structural and quantitative information useful for metabolomics fingerprinting, chemometric analysis, metabolite identification and metabolic pathway construction. A successful metabolomics study relies on proper experimental protocols for the collection, handling, processing and analysis of metabolomics data. Critically, these protocols should eliminate or avoid biologically-irrelevant changes to the metabolome. We provide a comprehensive description of our NMR-based metabolomics procedures optimized for the analysis of bacterial metabolomes. The technical details described within this manuscript should provide a useful guide to reliably apply our NMR-based metabolomics methodology to systems biology studies.

Indexed as

bacteriachemometricsmetabolomicsMycobacteriumNMRStaphylococcus

Identifiers

PMID26078915
PMCPMC4465129

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.