ArticleCell and tissue research2016
INSL3 stimulates spermatogonial differentiation in testis of adult zebrafish (Danio rerio).
Article in Cell and tissue research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 64 citations in OpenAlex.
- INSL3 Preserves Male Reproductive Function Under Inflammatory and Glucocorticoid Stress in Rats.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- Reception of conspecific cues alters testicular gene expression and improves fertility in boreal chorus frogs (Pseudacris maculata).Scientific reports · 2026Article
- Aging-TCA: a cross-species single-cell transcriptomic atlas for studying testicular aging.BMC genomics · 2025Article
- Cuproptosis and cuproptosis-related cell death and genes: mechanistic links to spermatogenic cell death.Cell death discovery · 2025Review
- The fourspine stickleback (Apeltes quadracus) has an XY sex chromosome system with polymorphic inversions on both X and Y chromosomes.PLoS genetics · 2025Article
- A century of andrology in Cell & Tissue Research: looking back while moving forward.Cell and tissue research · 2025Review
- Expression of Insl3 Protein in AdultInternational journal of molecular sciences · 2024Article
- Trpv4-mediated apoptosis of Leydig cells induced by high temperature regulates sperm development and motility in zebrafish.Communications biology · 2024Article
- Review
- Single Cell Transcriptome Sequencing of Zebrafish Testis Revealed Novel Spermatogenesis Marker Genes and Stronger Leydig-Germ Cell Paracrine Interactions.Frontiers in genetics · 2022Article
- Transcriptomes of testis and pituitary from male Nile tilapia (O. niloticus L.) in the context of social status.PloS one · 2022Article
- Evidence for existence of insulin-like factor 3 (INSL3) hormone-receptor system in the ovarian corpus luteum and extra-ovarian reproductive organs during pregnancy in goats.Cell and tissue research · 2021Article
- Insulin-like 3 affects zebrafish spermatogenic cells directly and via Sertoli cells.Communications biology · 2021Article
- Loss ofFrontiers in cell and developmental biology · 2021Article
- The G178A polymorphic variant of INSL3 may be linked to cryptorchidism among Egyptian pediatric cohort.Pediatric surgery international · 2020Article
- Spermatogonial Stem Cells in Fish: Characterization, Isolation, Enrichment, and Recent Advances of In Vitro Culture Systems.Biomolecules · 2020Review
- Entry into puberty is reflected in changes in hormone production but not in testicular receptor expression in Atlantic salmon (Salmo salar).Reproductive biology and endocrinology : RB&E · 2019Article
- The initiation of puberty in Atlantic salmon brings about large changes in testicular gene expression that are modulated by the energy status.BMC genomics · 2019Article
- Insulin-like peptide 3 expressed in the silkworm possesses intrinsic disulfide bonds and full biological activity.Scientific reports · 2017Article
- Distinct and Cooperative Roles ofGenetics · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
INSL3 (insulin-like peptide 3) is a relaxin peptide family member expressed by Leydig cells in the vertebrate testis. In mammals, INSL3 mediates testicular descent during embryogenesis but information on its function in adults is limited. In fish, the testes remain in the body cavity, although the insl3 gene is still expressed, suggesting yet undiscovered, evolutionary older functions. Anti-Müllerian hormone (Amh), in addition to inhibiting spermatogonial differentiation and androgen release, inhibits the Fsh (follicle-stimulating hormone)-induced increase in insl3 transcript levels in zebrafish testis. Therefore, the two growth factors might have antagonistic effects. We examine human INSL3 (hINSL3) effects on zebrafish germ cell proliferation/differentiation and androgen release by using a testis tissue culture system. hINSL3 increases the proliferation of type A undifferentiated (Aund) but not of type A differentiating (Adiff) spermatogonia, while reducing the proliferation of Sertoli cells associated with proliferating Aund. Since the area occupied by Aund decreases and that of Adiff increases, we conclude that hINSL3 recruits Aund into differentiation; this is supported by the hINSL3-induced down-regulation of nanos2 transcript levels, a marker of single Aund spermatogonia in zebrafish and other vertebrates. Pulse-chase experiments with a mitosis marker also indicate that hINSL3 promotes spermatogonial differentiation. However, hINSL3 does not modulate basal or Fsh-stimulated androgen release or growth factor transcript levels, including those of amh. Thus, hINSL3 seems to recruit Aund spermatogonia into differentiation, potentially mediating an Fsh effect on spermatogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.