ArticleThe Journal of membrane biology2015
Different Cell Viability Assays Reveal Inconsistent Results After Bleomycin Electrotransfer In Vitro.
Article in The Journal of membrane biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Water soluble curcumin with alkyl sulfonate moiety: Synthesis, and anticancer efficacy.Heliyon · 2024Article
- The equivalence of different types of electric pulses for electrochemotherapy with cisplatin - anRadiology and oncology · 2024Article
- Dynamics of Cell Death Due to Electroporation Using Different Pulse Parameters as Revealed by Different Viability Assays.Annals of biomedical engineering · 2024Article
- The Assessment of Calcium and Bleomycin Cytotoxic Efficiency in Relation to Cavitation Dosimetry.Pharmaceutics · 2023Article
- Exploring the Antimicrobial and Pharmacological Potential of NF22 as a Potent Inhibitor ofPharmaceutics · 2022Article
- Article
- Evaluation of Biological Activity of Natural Compounds: Current Trends and Methods.Molecules (Basel, Switzerland) · 2022Review
- Article
- The Evidence of the Bystander Effect after Bleomycin Electrotransfer and Irreversible Electroporation.Molecules (Basel, Switzerland) · 2021Article
- Sudden Cell Death Induced by CaBiomedicines · 2021Article
- Extracellular-CaPharmaceutics · 2020Article
- Optimization of cell viability assays to improve replicability and reproducibility of cancer drug sensitivity screens.Scientific reports · 2020Article
- A Novel Method for Controlled Gene Expression via Combined Bleomycin and Plasmid DNA Electrotransfer.International journal of molecular sciences · 2019Article
- The use of high-frequency short bipolar pulses in cisplatin electrochemotherapy in vitro.Radiology and oncology · 2019Article
- In Silico, Cytotoxic and Antioxidant Potential of Novel Ester, 3-hydroxyoctyl -5Drug design, development and therapy · 2019Article
- Comparison of cell-based assays to quantify treatment effects of anticancer drugs identifies a new application for Bodipy-L-cystine to measure apoptosis.Scientific reports · 2018Article
- A new electrospray method for targeted gene delivery.Scientific reports · 2018Article
- Calcein Release from Cells In Vitro via Reversible and Irreversible Electroporation.The Journal of membrane biology · 2018Article
- Cryopreservation of Human Adipose-Derived Stem Cells in Combination with Trehalose and Reversible Electroporation.The Journal of membrane biology · 2017Article
- Intracellular Delivery of Bleomycin by Combined Application of Electroporation and Sonoporation in Vitro.The Journal of membrane biology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to compare different and commonly used cell viability assays after CHO cells treatment with anticancer drug bleomycin (20 nM), high voltage (HV) electric pulses (4 pulses, 1200 V/cm, 100 µs, 1 Hz), and combination of bleomycin and HV electric pulses. Cell viability was measured using clonogenic assay, propidium iodide (PI) assay, MTT assay, and employing flow cytometry modality to precisely count cells in definite volume of the sample (flow cytometry assay). Results showed that although clonogenic cell viability drastically decreased correspondingly to 57 and 3 % after cell treatment either with HV pulses or combination of bleomycin and HV pulses (bleomycin electrotransfer), PI assay performed ~15 min after the treatments indicated nearly 100 % cell viability. MTT assay performed at 6-72 h time points after these treatments revealed that MTT cell viability is highly dependent on evaluation time point and decreased with later evaluation time points. Nevertheless, in comparison to clonogenic cell viability, MTT cell viability after bleomycin electrotransfer at all testing time points was significantly higher. Flow cytometry assay if used at later times, 2-3 days after the treatment, allowed reliable evaluation of cell viability. In overall, our results showed that in order to estimate cell viability after cell treatment with combination of the bleomycin and electroporation the most reliable method is clonogenic assay. Improper use of PI and MTT assays can lead to misinterpretation of the experimental results.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.