ArticlePlant physiology2015
Functional Divergence of Diterpene Syntheses in the Medicinal Plant Salvia miltiorrhiza.
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Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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80 citing papers in PubMed, 121 citations in OpenAlex.
- Biosynthesis and heterologous production of the α-agarofuran scaffold of Celangulin V from Celastrus angulatus.Nature communications · 2026Article
- A Review of Plant-Derived Diterpenoid Biosynthesis: From Structural Scaffold Diversity and Lineage-Associated Distribution to Enzyme Mining and Discovery Strategies.Molecules (Basel, Switzerland) · 2026Review
- Divergent Roles of SmHMGR2 and a Novel SmHMGR5 in Tanshinone Biosynthesis Revealed by CRISPR/Cas9-Mediated Knockout inInternational journal of molecular sciences · 2026Article
- The transcriptional regulatory network involved in periderm-specific tanshinones biosynthesis inActa pharmaceutica Sinica. B · 2026Article
- The Jasmonate-Responsive SmMPK3-SmWRKY33 Module Positively Regulates Tanshinone Biosynthesis in Salvia miltiorrhiza.Plant biotechnology journal · 2026Article
- Functional identification of two cytochrome P450 enzymes involved in diterpenoid oxidation inFrontiers in plant science · 2026Article
- CRISPR/Cas9-mediated uORF engineering enhances tanshinone biosynthesis inHorticulture research · 2026Article
- Quantitative Trait Locus Mapping and Candidate Gene Analysis of the Contents of Three Tanshinone Components in Salvia miltiorrhiza Bunge.Biochemical genetics · 2025Article
- SmbZIP5 Integrates Abscisic and Jasmonic Acid Signalling to Positively Regulate Tanshinone Biosynthesis in Salvia miltiorrhiza.Plant biotechnology journal · 2025Article
- Molecular mechanism ofHorticulture research · 2025Article
- Genome-wide characterization and expression analysis of WRKY family genes in the biosynthesis of triptolide in Tripterygium wilfordii.BMC genomics · 2025Article
- Genome-wide identification of the SmPHR gene family in Salvia miltiorrhiza and SmPHR7-mediated response to phosphate starvation in Arabidopsis thaliana.Plant cell reports · 2025Article
- UHPLC-QTOF-MS-Based Targeted Metabolomics Provides Novel Insights into the Accumulative Mechanism of Soil Types on the Bioactive Components ofMolecules (Basel, Switzerland) · 2024Article
- Biosynthesis and signal transduction of plant growth regulators and their effects on bioactive compound production in Salvia miltiorrhiza (Danshen).Chinese medicine · 2024Review
- From functional plasticity of two diterpene synthases (IrTPS2/IrKSL3a) to enzyme evolution.ACS catalysis · 2024Article
- Comparative transcriptomics of two Salvia subg. Perovskia species contribute towards molecular background of abietane-type diterpenoid biosynthesis.Scientific reports · 2024Article
- Sequence-Structure Analysis Unlocking the Potential Functional Application of the Local 3D Motifs of Plant-Derived Diterpene Synthases.Biomolecules · 2024Article
- Application of metabolomics in quality control of traditional Chinese medicines: a review.Frontiers in plant science · 2024Review
- Enhancement of specialized metabolites using CRISPR/Cas gene editing technology in medicinal plants.Frontiers in plant science · 2024Review
- Functions of Representative Terpenoids and Their Biosynthesis Mechanisms in Medicinal Plants.Biomolecules · 2023Review
20 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 4 institutions in 2 countries.
Guanghong CuiKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (G.C., L.D., Z.X., G.S., J.H.S., X.Q.);State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China (G.C., B.J., L.H.);Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China (J.Q., J.S., S.C.); andDepartment of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (R.J.P.).
Lixin DuanKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (G.C., L.D., Z.X., G.S., J.H.S., X.Q.);State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China (G.C., B.J., L.H.);Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China (J.Q., J.S., S.C.); andDepartment of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (R.J.P.).
Baolong JinKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (G.C., L.D., Z.X., G.S., J.H.S., X.Q.);State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China (G.C., B.J., L.H.);Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China (J.Q., J.S., S.C.); andDepartment of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (R.J.P.).
Jun QianKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (G.C., L.D., Z.X., G.S., J.H.S., X.Q.);State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China (G.C., B.J., L.H.);Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China (J.Q., J.S., S.C.); andDepartment of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (R.J.P.).
Zheyong XueKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (G.C., L.D., Z.X., G.S., J.H.S., X.Q.);State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China (G.C., B.J., L.H.);Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China (J.Q., J.S., S.C.); andDepartment of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (R.J.P.).
Guoan ShenKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (G.C., L.D., Z.X., G.S., J.H.S., X.Q.);State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China (G.C., B.J., L.H.);Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China (J.Q., J.S., S.C.); andDepartment of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (R.J.P.).
John Hugh SnyderKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (G.C., L.D., Z.X., G.S., J.H.S., X.Q.);State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China (G.C., B.J., L.H.);Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China (J.Q., J.S., S.C.); andDepartment of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (R.J.P.).
Jingyuan SongKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (G.C., L.D., Z.X., G.S., J.H.S., X.Q.);State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China (G.C., B.J., L.H.);Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China (J.Q., J.S., S.C.); andDepartment of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (R.J.P.).
Shilin ChenKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (G.C., L.D., Z.X., G.S., J.H.S., X.Q.);State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China (G.C., B.J., L.H.);Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China (J.Q., J.S., S.C.); andDepartment of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (R.J.P.).
Luqi HuangKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (G.C., L.D., Z.X., G.S., J.H.S., X.Q.);State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China (G.C., B.J., L.H.);Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China (J.Q., J.S., S.C.); andDepartment of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (R.J.P.).
Reuben J PetersKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (G.C., L.D., Z.X., G.S., J.H.S., X.Q.);State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China (G.C., B.J., L.H.);Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China (J.Q., J.S., S.C.); andDepartment of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (R.J.P.) xqi@ibcas.ac.cn rjpeters@iastate.edu.ORCID http://orcid.org/0000-0003-4691-8477
Xiaoquan QiKey Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (G.C., L.D., Z.X., G.S., J.H.S., X.Q.);State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China (G.C., B.J., L.H.);Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China (J.Q., J.S., S.C.); andDepartment of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011 (R.J.P.) xqi@ibcas.ac.cn rjpeters@iastate.edu.ORCID http://orcid.org/0000-0001-7175-115X
Chinese Academy of Medical Sciences & Peking Union Medical College · CNInstitute of Botany · CNChinese Academy of Sciences · CNIowa State University · US
Funding
Enzymatic formation of the hydrocarbon skeletons underlying diterpenoid diversityR01GM076324 · NIGMS · IOWA STATE UNIVERSITY · PI PETERS, REUBEN JOHN · 2006 to 2019
$3.4MNIGMS NIH HHS GM076324NIGMS NIH HHS R01 GM076324
Abstract
The medicinal plant Salvia miltiorrhiza produces various tanshinone diterpenoids that have pharmacological activities such as vasorelaxation against ischemia reperfusion injury and antiarrhythmic effects. Their biosynthesis is initiated from the general diterpenoid precursor (E,E,E)-geranylgeranyl diphosphate by sequential reactions catalyzed by copalyl diphosphate synthase (CPS) and kaurene synthase-like cyclases. Here, we report characterization of these enzymatic families from S. miltiorrhiza, which has led to the identification of unique pathways, including roles for separate CPSs in tanshinone production in roots versus aerial tissues (SmCPS1 and SmCPS2, respectively) as well as the unique production of ent-13-epi-manoyl oxide by SmCPS4 and S. miltiorrhiza kaurene synthase-like2 in floral sepals. The conserved SmCPS5 is involved in gibberellin plant hormone biosynthesis. Down-regulation of SmCPS1 by RNA interference resulted in substantial reduction of tanshinones, and metabolomics analysis revealed 21 potential intermediates, indicating a complex network for tanshinone metabolism defined by certain key biosynthetic steps. Notably, the correlation between conservation pattern and stereochemical product outcome of the CPSs observed here suggests a degree of correlation that, especially when combined with the identity of certain key residues, may be predictive. Accordingly, this study provides molecular insights into the evolutionary diversification of functional diterpenoids in plants.
Indexed as
DiterpenesGene Expression Regulation, PlantMolecular StructurePhylogenyPlant ProteinsRecombinant ProteinsRNA InterferenceSalvia miltiorrhizaTranscriptomeDiterpenesPlant ProteinsRecombinant Proteins
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