ArticleAnalytical and bioanalytical chemistry2015
Facile synthesis of deuterium-labeled denatonium cation and its application in the quantitative analysis of Bitrex by liquid chromatography-mass spectrometry.
Article in Analytical and bioanalytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 19 citations in OpenAlex.
- Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics.Metabolites · 2023Review
- Peptidyl-Resin Substrates as a Tool in the Analysis of Caspase Activity.Molecules (Basel, Switzerland) · 2022Article
- Preparation of Deuterium-Labeled Armodafinil by Hydrogen-Deuterium Exchange and Its Application in Quantitative Analysis by LC-MS.Metabolites · 2022Article
- A Non-Covalent Dimer Formation of Quaternary Ammonium Cation with Unusual Charge Neutralization in Electrospray-Ionization Mass Spectrometry.Molecules (Basel, Switzerland) · 2021Article
- Trends in the Hydrogen-Deuterium Exchange at the Carbon Centers. Preparation of Internal Standards for Quantitative Analysis by LC-MS.Molecules (Basel, Switzerland) · 2021Review
- Preparation of Isotopically Labelled Standards of Creatinine Via H/D Exchange and Their Application in Quantitative Analysis by LC-MS.Molecules (Basel, Switzerland) · 2020Article
- Trends in the Design of New Isobaric Labeling Reagents for Quantitative Proteomics.Molecules (Basel, Switzerland) · 2019Review
- Rapid Assessment of Contaminants and Interferences in Mass Spectrometry Data Using Skyline.Journal of the American Society for Mass Spectrometry · 2018Article
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4 authors at 1 institution in 1 country.
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Abstract
Quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) is frequently based on the application of isotopically labeled standards which usually have to be specially synthesized. Although the synthesis of deuterated isotopologues is relatively inexpensive, they are not considered as good internal standards due to the possible deuterium effect on the retention time during LC-MS analysis. We developed a method of deuterium labeling of denatonium benzoate (Bitrex) via H/D exchange of its α-carbon hydrogen atoms in CH2 group situated between carbonyl and quaternary ammonium groups. The proposed strategy is rapid, cost-efficient, and does not require derivatization reagents or further purification. The LC-MS analysis of isotopologues revealed that the introduced deuterons do not undergo back exchange under acidic conditions, and the co-elution of deuterated and non-deuterated forms is observed. The obtained deuterated standard was applied in the quantitative LC-MS analysis of Bitrex in commercially available household products.
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